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Published Articles from USDA ARS National Program 108: Food Safety
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Title: Rapid sample preparation and fast GC-MS/MS for the analysis of pesticides and environmental contaminants in fish
Authors: Sapozhnikova Y
Journal: LC GC N Am
Accepted date: 2014 Mar 18
Interpretive summary: In this study we compared efficiency of the three sorbents for d-SPE clean-up of fish extracts for the analysis of pesticides and environmental contaminants: C18+PSA, Z-Sep and Z-Sep Plus. C18+PSA removed the highest amounts of co-extractive materials by weight (80%), followed by Z-Sep (66%) and Z-Sep Plus (55%). In terms of recoveries of target analytes, Z-sep provided the highest recoveries for most of the contaminants, and Z-sep Plus showed greatest standard deviations, perhaps, caused by matrix interferences. Recoveries of analytes not normalized to ISTDs, were 2-3 times lower for all classes, except for pesticides, reflecting the loss of the corresponding ISTDs during the sample preparation. Two major co-extractive compounds identified in the extracts that contributed to MEs were oleic and octadecanoic acid, and d-SPE with Z-Sep removed these compounds most effectively. Peak shapes and widths were altered by co-eluting matrix interferences as we observed in the case of o,p’-DDE. Calculated MEs for TPP and o,p’-DDE based on peak areas were lowest for d-SPE with C18+PSA, followed by Z-Sep and Z-Sep Plus, however they were less than 3% for all the treatments when normalized to ISTDs, indicating the need of using ISTD for ME compensation. Finally, Pearson correlations were reported for compounds’ recoveries, tR and log Kow values.
Publication date: 2014 Nov
Pages: 878-86