Title: Determination of deoxynivalenol in bran and whole-wheat flour by fluorescence polarization immunoassay
Authors: Valenzano S, Lippolis V, Pascale M, DeMarco C, Maragos C,Suman M,Visconti A
Journal: J Food Anal Methods
Accepted date: 2013 Jul 7
Interpretive summary: Many countries have problems with naturally occurring toxins in grains. One such toxin is deoxynivalenol (DON), which is commonly found in wheat, barley, and corn. When grains are processed, DON does not distribute equally among the fractions. The increasing inclusion of bran into food formulations, as a source of dietary fiber, necessitates increased monitoring for DON in such fractions. As part of efforts to improve monitoring of the toxin, a method for detecting DON in wheat bran and whole-wheat flour using fluorescence polarization immunoassay was developed at the Institute of Sciences of Food Production (ISPA) in Bari, Italy, with help from ARS scientists in Peoria, IL. The technique was successfully used to measure DON in samples of naturally contaminated bran and whole-wheat flours, with results that agreed well with a reference method. The result is a rapid method that can be used to monitor DON in wheat bran and whole-wheat flour.
Publication date: 2014 Apr
Related projects: Innovative Materials for Use in Mycotoxin Detection