Title: Production of anti-idiotype antibodies for deoxynivalenol and their evaluation with three immunoassay platforms
Authors: Maragos CM
Journal: Mycotoxin Res
Accepted date: 2014 Jan 17
Interpretive summary: Deoxynivalenol (DON) is a toxin produced by certain species of fungi that can infest wheat, barley, and corn worldwide. Because of this the U.S. conducts extensive monitoring of commodities. As part of efforts to improve monitoring of the toxin, kits for detecting DON based upon specific antibodies (immunoassays) have been widely developed. In such kits, the toxin is generally included as a standard against which test samples are compared. This means that the toxin is shipped with the test kits, and that analysts have the potential to be exposed to the toxin when the kits are used. However, materials other than the toxin can potentially be used, provided they mimic the toxin in the assay. Scientists with the Bacterial Foodborne Pathogens and Mycology research unit in Peoria, IL, developed a material (an anti-idiotype antibody) that mimics the effects of the toxin in assays and evaluated the material in three immunoassay formats. This material has the potential to replace the toxin in diagnostic test kits, which would make the kits safer to use and to ship.
Publication date: 2014 Feb 13
Related projects: Innovative Materials for Use in Mycotoxin Detection