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Published Articles from USDA ARS National Program 108: Food Safety
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Title: An enzyme-linked immunosorbent assay for determination of dicyclanil in animal tissue
Authors: Hou X, Wang J, Sun Y, Lu Y, Beier RC
Journal: Analyt Lett
Accepted date: 2013 Jun 9
Interpretive summary: Dicyclanil is an insect growth regulator used in veterinary medicine for the prevention of myiasis or fly-strike, a disease resulting from the infestation of tissue by fly larvae, in Europe, the Middle East, and Africa. Residues of dicyclanil in food animal tissue may be a food safety issue since dicyclanil-induced hepatocarcinogenesis has been observed in mice. This paper demonstrates the development of an enzyme-linked immunosorbent assay (ELISA) using polyclonal antibodies from rabbits for the determination of dicyclanil residues in sheep muscle, liver, kidney, and in milk. Antibodies are substances that are produced by the immune system in response to foreign substances which enter the body. Once the antibodies to a foreign substance are isolated, they can be used in a method to detect the presence of that foreign substance. The homogenized meat samples and milk samples were extracted, and the extracts were analyzed with the ELISA. The mean recoveries of dicyclanil were between 52.5% and 72.4%. The developed dicyclanil analysis method was rapid, sensitive, and reliable. The results demonstrated that the developed method can be used as a screening method to quantify dicyclanil residues in animal tissues.
Publication date: 2013 Nov
Volume: 46
Issue: 18
Pages: 2870-8