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Published Articles from USDA ARS National Program 108: Food Safety
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Title: Surface display of Bombyx mori acetylcholinesterase on Pichia pastoris and its bioactivity study
Authors: Dong J, Xie X, He Y, Beier RC, Sun Y, Xu Z, Wu W, Shen Y, Xiao Z, Lai L, Wang H, Yang J
Journal: PLoS One
Accepted date: 2013 Jun 19
Interpretive summary: Acetylcholinesterase (AChE) is a key enzyme because it regulates the level of acetylcholine in the blood and terminates nerve impulses. Carbamate (CB) and organophosphorous (OP) pesticides act to paralyze and kill insects by inhibiting their AchE enzyme. Residues of these pesticides may be found in food and the environment. It is feasible to determine the content of CB and OP pesticides according to the degree of inhibition of AChE activity. In this study, we used modern molecular biology tools to facilitate development of a rapid, easy, and sensitive analytical method useful for the detection of anticholinesterase pesticide residues in food and the environment.
Publication date: 2013 Aug 5
Volume: 8
Issue: 8
Pages: e70451