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Published Articles from USDA ARS National Program 108: Food Safety
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Title: The potential use of bacteria community succession in forensics as described by high throughput metagenomic sequencing
Authors: Pechal JL, Crippen TL, Benbow ME, Tarone AM, Dowd S, Tomberlin JK
Journal: Int J Legal Med
Accepted date: 2013 May 7
Interpretive summary: Decomposition studies often focus on data that can be seen with the naked eye, such as insects and animal scavengers, and ignore the bacterial communities present. We documented the bacterial species present during decomposition of animal carrion. High throughput genetic sequence identification was used to describe abundance and types of bacteria at specific time points during the decay process. This information is important to understanding locations and movement of bacteria and bacterial pathogens on animal carcasses. The bacterial community showed differences in composition and abundance over time. A model was developed using this genetic technique that provides a framework for standard operating procedure of how to use high throughput sequencing as a potential new tool to document and identify bacterial communities during decomposition. This tool is important to animal production operations to monitor the presence and movement of pathogens during normal operations and during mass die-offs associated with man-made and natural disasters and disease epidemics.
Publication date: 2014 Jan
Volume: 128
Issue: 1
Pages: 193-205