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Detection and Typing of Food-Borne Pathogens

Develop protein- and nucleic acid-based methods for detection, and characterization of food-borne pathogens. Specifically, develop antibody- and oligonucleotide-based detection microarrays for multiple pathogens; and improved real-time multiplex PCR assays. Develop rapid and effective means to separate and concentrate targeted pathogens from food matrices that can be coupled to very rapid detection methods such as real-time PCR. Develop molecular typing methods for pathogens of concern to associated food regulatory agencies. Examine environmental factors and microbiological culture conditions affecting genotypes or phenotypes that are important for virulence, isolation, or detection of foodborne pathogens.
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This project will undertake the development of technologies for the detection and characterization of foodborne bacterial pathogens of critical concern. Molecular characterization and typing methods will be generated for the most common foodborne pathogens including E. coli and Salmonella species. Analysis will center on antibody and nucleic acid microarray platforms as supported by co-development of highly effective bacterial concentration, enrichment, isolation and/or separation methods. Emerging technologies will be directed towards usage by food producers and food regulatory agencies for food safety monitoring and follow-up investigations.
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Bhaduri, Saumya
Brewster, Jeffrey
Gehring, Andrew
He, Yiping
Paoli, George
Published Journal Articles USDA ARS (NP 108):
DNA extraction protocol for rapid PCR detection of pathogenic bacteria
Brewster JD, Paoli GC .
Anal Biochem. 2013 Nov 1;442(1):107-9.
Antibacterial activity and mechanism of action of zinc oxide nanoparticles against Campylobacter jejuni
Xie Y, He Y, Irwin PL, Jin T, Shi X.
Appl Environ Microbiol. 2011 Apr;77(7):2325-31.
High-throughput biosensors for multiplexed food-borne pathogen detection
Gehring AG, Tu SI.
Annu Rev Anal Chem (Palo Alto Calif). 2011;4:151-72.