Food Safety Research Information Office
Title:Interventions and Methodologies to Reduce Human Food-borne Bacterial Pathogens in Chickens
Objective:The purpose of the Poultry Microbiological Safety Research Unit is to further reduce or eliminate bacterial pathogen contamination in poultry operations and the following objectives are to: 1. Assess the effectiveness and further development of bacteriocins (anti-bacterial peptides) and bacteriophage by in vitro bacterial growth inhibition in culture and in vivo experimentation via challenge in chickens. 2. Reduce bacterial populations in chicken litter by monitoring poultry houses for bacterial pathogens carried by chickens during use of intervention technologies. Decontamination techniques, such as in-house foam applied disinfectants will be examined for reduction of Campylobacter spp., Salmonella spp., Clostridium perfringens and Listeria monocytogenes. 3. Improve cultural methods for Campylobacter spp. in poultry in further support of FSIS needs, including the improvement of recovery. Use microarray expression analysis under various cultural conditions to identify nutrients necessary for optimal growth, colonization and culture of Campylobacter spp.
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Approach: New antimicrobial agents will be developed that are usable by the poultry farmer on a large scale to reduce Campylobacter spp. and salmonellae by applying in feed at the appropriate times before slaughter. Also, new means for the drug-free production sector to control clostridial disease without relying on antibiotics are the major expected approaches of Objective 1. Providing a science based source of data to better dispose of spent poultry litter without major energy or financial inputs is the approach for Objective 2. A more reliable and quantitative method to routinely culture Campylobacter spp. from food and environmental samples is the approach of Objective 3.
Funding Source:United States Department of Agriculture (USDA), Agricultural Research Service (ARS)
Type:Appropriated
Start Date:2011
End Date:2012
Project Number:6612-32000-061-00
Accession Number:421064
Institutions:USDA/ARS - South Atlantic Area
Investigators:Buhr, Richard
Cox, Nelson
Hiett, Kelli
Line, John
Seal, Bruce
Published Journal
Articles USDA
ARS (NP 108):
Variations in preenrichment pH of poultry feed and feed ingredients after incubation periods up to 48 hours
Cox Jr NA, Cason JA, Buhr RJ, Richardson KE, Richardson LJ, Rigsby LL, Cray PJ.
J Appl Poultry Res. 2013 Jun;22(2):190-5.
Efficacy of combination chemicals as sanitizers of Salmonella-inoculated broiler hatching eggshells
Buhr RJ, Spickler JL, Ritter AR, Bourassa DV, Cox NA, Richardson LJ, Wilson JL.
J Appl Poult Res. 2013;22(1):27-35.
Non-Journal Publications:
Salmonella - (Book / Chapter)
(03-Feb-13)
Application of Removable Coatings and Storage Time on Internal Quality of Broiler Hatching Eggs - (Abstract Only)
Mauldin, J.M., Buhr, R.J., Jones, D.R., Santos, J., Aggrey, S.E. 2012. Application of Removable Coatings and Storage Time on Internal Quality of Broiler Hatching Eggs 91(Suppl. 1):167. p.91
Sampling each naturally contaminated broiler carcass for Salmonella by three different methods - (Abstract Only)
Cox Jr, N.A., Buhr, R.J., Cason, J.A., Cray, P.J., Rigsby, L.L., Bourassa, D.V., Cosby, D.E. 2012. Sampling each naturally contaminated broiler carcass for Salmonella by three different methods. Poultry Science. 91(Suppl. 1):155-156.
Survival of naturally occurring Campylobacter in refrigerated and frozen rinsate from a broiler carcass - (Abstract Only)
Cox Jr, N.A., Larry, R.J., Berrang, M.E., Rigsby, L.L., Buhr, R.J., Plumblee, J., Cray, P.J. 2012. Survival of naturally occurring Campylobacter in refrigerated and frozen rinsate from a broiler carcass. Poultry Science. 91(Suppl. 1):156.
Impact of broiler slaughter scalding method on processing wastewater stream - (Abstract Only)
Kiepper, B.H., Buhr, R.J., Walker, J.M., Bourassa, D.V., Brooke, C.A., Zhuang, H. 2012. Impact of broiler slaughter scalding method on processing wastewater stream. Poultry Science Meeting. 91(suppl. 1):69. p.25.
Impact of broiler processing scalding and chilling profiles on carcass and breast meat yield - (Abstract Only)
Buhr, R.J., Walker, J.M., Bourassa, D.V., Caudill, B.A., Kiepper, B.H., Zhuang, H. 2012. Impact of broiler processing scalding and chilling profiles on carcass and breast meat yield. Poultry Science Meeting. Poultry Science 91:(Suppl.1)68.p.25.2012.
Clearance of Escherichia coli After Intravenous Inoculation in Broiler Breeder Pullets Fed Skip a day, Every Day in the feeder and Every Day on the Litter - (Abstract Only)
Montiel, E.R., Buhr, R.J., Cox Jr, N.A., Hofacre, C.L., Davis, A.J., Wilson, J.L. 2012. Clearance of Escherichia coli After Intravenous Inoculation in Broiler Breeder Pullets Fed Skip a day, Every Day in the feeder and Every Day on the Litter. Poultry Science Meeting. 91(Suppl. 1):18. p.8.
Impact of Skip a Day and Every Day Feeding Programs on the Colonization Rates of Salmonella Enteritidis in Broiler Breeder Pullets - (Abstract Only)
Montiel, E.R., Buhr, R.J., Cox Jr, N.A., Hofacre, C.L., Davis, A.J., Wilson, J.L. 2012. Impact of Skip a Day and Every Day Feeding Programs on the Colonization Rates of Salmonella Enteritidis in Broiler Breeder Pullets. Poultry Science Meeting. 91(Suppl. 1):17. p7-8.
Food Safety Categories:Food and Feed Composition and Characteristics
On-Farm Food Safety
Methodology
Farm-to-Table Categories:On-farm food production
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