|Title:||Genetic Analysis of Poultry-Associated Salmonella Enterica to Identify & Characterize Properties & Markers Associated with Egg-Borne Transmission|
|Objective:||1. Use the Salmonella Enteritidis (SE) SNP database to link specific genes and regulatory circuits to phenotypes associated with egg contamination, virulence, pathogenicity, host preference, or environmental persistence of subpopulations. |
2. Determine whether recurring subsets or patterns of SE SNPs can be identified that are causally linked to outbreak potential of other Salmonella serotypes, and determine if SNPs can be used for risk profiling and cluster detection.
3. Apply information about genomic variation occurring across Salmonella enterica to improve serotyping schemes and vaccines that can be used to develop rapid and sensitive diagnostic and epidemiological testing methodologies for SE in poultry flocks and eggs.
|More Info:||Approach: Sixteen genes have been identified that are disrupted by SNPS SNPs in strains that vary in virulence potential. These genes will be mutated in a parent strain with a characterized genome and will be assayed by phenotype microarray and hen infection studies for function in virulence pathways. New SNPs will be identified that differentiate prominent phage types that vary in geographical incidence using whole genome comparisons. SNPs that have been linked to virulence or geographical incidence will be analyzed across other Salmonella serotypes by primer-directed sequencing for the purpose of evaluating epidemiological trends associated with SNPs.|
|Funding Source:||United States Department of Agriculture (USDA), Agricultural Research Service (ARS)|
|Institutions:||USDA/ARS - South Atlantic Area|
|Project Reports:||2012 Annual Report|
2011 Annual Report
|Pending USDA ARS Publications (NP 108):|
ARS (NP 108):
Assignment of serotype to Salmonella enterica isolates obtained from poultry and their environment in southern Brazil
Pulido-Landínez M, Sánchez-Ingunza R, Guard J, Nascimento VP.
Lett Appl Microbiol. 2013 Oct;57(4):288-94.
Comparison of dkgB-linked intergenic sequence ribotyping to DNA microarray hybridization for assigning serotype to Salmonella enterica
Guard J, Sanchez-Ingunza R, Morales C, Stewart T, Liljebjelke K, Van Kessel J, Ingram K, Jones D, Jackson C, Fedorka-Cray P, Frye J, Gast R, Hinton A Jr.
FEMS Microbiol Lett. 2012 Dec;337(1):61-72.
Transposon mutagenesis of Salmonella enterica serovar Enteritidis identifies genes that contribute to invasiveness in human and chicken cells and survival in egg albumen
Shah DH, Zhou X, Kim HY, Call DR, Guard J.
Infect Immun. 2012 Dec;80(12):4203-15.
Virulence and metabolic characteristics of Salmonella enterica serovar enteritidis strains with different sefD variants in hens
Morales CA, Guard J, Sanchez-Ingunza R, Shah DH, Harrison M.
Appl Environ Microbiol. 2012 Sep;78(18):6405-12.
Salmonella Enteritidis strains from poultry exhibit differential responses to acid stress, oxidative stress, and survival in the egg albumen
Shah DH, Casavant C, Hawley Q, Addwebi T, Call DR, Guard J.
Foodborne Pathog Dis. 2012 Mar;9(3):258-64.
Use of FTA cards for the transport of DNA samples of Salmonella spp. from poultry products from Southern Brazil
Pulido-Landínez M, Laviniki V, Sánchez-Ingunza R, Guard J, Nascimento VP.
Acta Sci Vet. 2012;40(4):1073.
Visible/NIR spectroscopy for Identifying Salmonella Infected Broilers - (Proceedings)
Accepted Publication (15-Aug-11)
Intergenic Sequence Ribotyping using a region neighboring dkgB links genovar to Kauffman-White serotype of Salmonella enterica - (Other)
Guard, J.Y. 2011. Intergenic Sequence Ribotyping using a region neighboring dkgB links genovar to Kauffman-White serotype of Salmonella enterica. National Center for Biotechnology Information (NCBI).
Hypothesis: A role for the mouse as an amplifier of Salmonella enterica on-farm - (Proceedings)
Guard, J.Y., Sanchez-Ingunza, R., Ahmer, B., Mcclelland, M., Henzler, D. 2011. Hypothesis: A role for the mouse as an amplifier of Salmonella enterica on-farm. Poultry Science Association, July 16, 2011, St. Louis, Mo. p.24.
Improving Food Safety by Understanding the Evolution of Egg-contaminating Salmonella Enteritidis - (Proceedings)
Guard, J.Y. 2011. Improving Food Safety by Understanding the Evolution of Egg-contaminating Salmonella Enteritidis. In: Proceedings of XXX International Seminar of AMEVEA. April 6-8, 2011, Bogota, Columbia. 2011CDROM.
|Food Safety Categories:||Contaminants and Contamination|
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