Food Safety Research Information Office
Title:Genetic Analysis of Poultry-Associated Salmonella Enterica to Identify & Characterize Properties & Markers Associated with Egg-Borne Transmission of Illness
Objective:1. Use the Salmonella Enteritidis (SE) SNP database to link specific genes and regulatory circuits to phenotypes associated with egg contamination, virulence, pathogenicity, host preference, or environmental persistence of subpopulations.

2. Determine whether recurring subsets or patterns of SE SNPs can be identified that are causally linked to outbreak potential of other Salmonella serotypes, and determine if SNPs can be used for risk profiling and cluster detection.

3. Apply information about genomic variation occurring across Salmonella enterica to improve serotyping schemes and vaccines that can be used to develop rapid and sensitive diagnostic and epidemiological testing methodologies for SE in poultry flocks and eggs.

More Info:
Approach: Sixteen genes have been identified that are disrupted by SNPS SNPs in strains that vary in virulence potential. These genes will be mutated in a parent strain with a characterized genome and will be assayed by phenotype microarray and hen infection studies for function in virulence pathways. New SNPs will be identified that differentiate prominent phage types that vary in geographical incidence using whole genome comparisons. SNPs that have been linked to virulence or geographical incidence will be analyzed across other Salmonella serotypes by primer-directed sequencing for the purpose of evaluating epidemiological trends associated with SNPs.
Funding Source:United States Department of Agriculture (USDA), Agricultural Research Service (ARS)
Type:Appropriated
Start Date:2011
End Date:2016
Project Number:6612-32000-007-00
Accession Number:420979
Institutions:USDA/ARS - South Atlantic Area
Investigators:Guard, Jean
Project Reports:2013 Annual Report
2012 Annual Report
2011 Annual Report
Published Journal
Articles USDA
ARS (NP 108):
Serotype and antimicrobial resistance patterns of Salmonella isolates from commercial birds and poultry environment in Mississippi
Pulido-Landinez M, Washington P, Thornton JK, Zhang Y, Sanchez-Ingunza R, Banda A, Guard J, Nascimento VP, Magee DL, Mauel MJ.
Avian Dis. 2014 Mar;58(1):64-70.
Presence of Salmonella enteritidis and Salmonella gallinarum in commercial laying hens diagnosed with fowl typhoid disease in Colombia
Pulido-Landínez M, Sánchez-Ingunza R, Guard J, do Nascimento VP.
Avian Dis. 2014 Mar;58(1):165-70.
Serotyping and determination of antimicrobial resistance patterns of Salmonella isolates from commercial birds and poultry environment in Mississippi
Pulido-Landinez M, Washington P, Thornton J, Zhang Y, Sanchez-Ingunza R, Banda A, Guard JY, Pinheiro Do Nascimen T, Magee D, Mauel MJ .
Avian Dis. 2014;58(1):64-70.
Dimethyl adenosine transferase (KsgA) deficiency in Salmonella enterica Serovar Enteritidis confers susceptibility to high osmolarity and virulence attenuation in chickens
Chiok KL, Addwebi T, Guard J, Shah DH.
Appl Environ Microbiol. 2013 Dec;79(24):7857-66.
Assignment of serotype to Salmonella enterica isolates obtained from poultry and their environment in southern Brazil
Pulido-Landínez M, Sánchez-Ingunza R, Guard J, Nascimento VP.
Lett Appl Microbiol. 2013 Oct;57(4):288-94.
Comparison of dkgB-linked intergenic sequence ribotyping to DNA microarray hybridization for assigning serotype to Salmonella enterica
Guard J, Sanchez-Ingunza R, Morales C, Stewart T, Liljebjelke K, Van Kessel J, Ingram K, Jones D, Jackson C, Fedorka-Cray P, Frye J, Gast R, Hinton A Jr.
FEMS Microbiol Lett. 2012 Dec;337(1):61-72.
Transposon mutagenesis of Salmonella enterica serovar Enteritidis identifies genes that contribute to invasiveness in human and chicken cells and survival in egg albumen
Shah DH, Zhou X, Kim HY, Call DR, Guard J.
Infect Immun. 2012 Dec;80(12):4203-15.
Virulence and metabolic characteristics of Salmonella enterica serovar enteritidis strains with different sefD variants in hens
Morales CA, Guard J, Sanchez-Ingunza R, Shah DH, Harrison M.
Appl Environ Microbiol. 2012 Sep;78(18):6405-12.
Salmonella Enteritidis strains from poultry exhibit differential responses to acid stress, oxidative stress, and survival in the egg albumen
Shah DH, Casavant C, Hawley Q, Addwebi T, Call DR, Guard J.
Foodborne Pathog Dis. 2012 Mar;9(3):258-64.
Use of FTA cards for the transport of DNA samples of Salmonella spp. from poultry products from Southern Brazil
Pulido-Landínez M, Laviniki V, Sánchez-Ingunza R, Guard J, Nascimento VP.
Acta Sci Vet. 2012;40(4):1073.
Cell invasion of poultry-associated Salmonella enterica serovar Enteritidis isolates is associated with pathogenicity, motility and proteins secreted by the type III secretion system
Shah DH, Zhou X, Addwebi T, Davis MA, Orfe L, Call DR, Guard J, Besser TE.
Microbiology. 2011 May;157(5):1428-45.
Non-Journal Publications:
Integrative Analysis of Salmonellosis Outbreaks in Israel 1999-2012 Revealed an Invasive S. enterica Serovar 9,12:l,v:- and Endemic S. Typhimurium DT104 strain - (Peer Reviewed Journal)
(29-Mar-14)
Development and application of novel SNP-based serotyping assays in targeting Salmonella enterica within the poultry production and processing continuum. - (Abstract Only)
(05-Mar-14)
Serotyping of Salmonella Isolates from Broiler Vertical Integrations in Colombia - (Abstract Only)
(27-Nov-13)
Development of a rapid serotyping method for Salmonella enterica using serotype-specific single-nucleotide polymorphisms - (Abstract Only)
(16-Nov-13)
Evolutionary relationships between known serotypes and unique variants of Salmonella enterica as determined by ISR secondary structure - (Abstract Only)
Guard, J.Y., Desai, P., Mcclelland, M. 2014. Evolutionary relationships between known serotypes and unique variants of Salmonella enterica as determined by ISR secondary structure. International Poultry Scientific Forum. p. 5.
Identification of risk factors and causes of persistence of Salmonella Gallinarum in laying hens farms from Colombia - (Abstract Only)
Pulido-Landinez, M., Banda, A., Guard, J.Y., Pinheironascimen, V.D. 2014. Identification of risk factors and causes of persistence of Salmonella Gallinarum in laying hens farms from Colombia. International Poultry Scientific Forum. pg. 6.
The prevalence of Salmonella enterica serovars Heidelberg and Kentucky in the scalder water of a commercial poultry processing plant in the southeastern United States. - (Abstract Only)
(06-Nov-13)
Population structure of Salmonella enterica subspecies enterica (subspecies 1) - (Abstract Only)
Desai, P.T., Porwollik, S., Long, F., Cheng, P., Weinstock, G.M., Fields, P., Weimer, B., Guiney, D., Gal-Mor, O., Rabsch, W., Guard, J.Y., Frye, J.G., Mcclelland, M. 2013. Population structure of Salmonella enterica subspecies enterica (subspecies 1). American Society for Microbiology Annual Meeting. p. 25.
Evaluation of a dkgB linked intergenic sequence ribotyping (ISR) method for assigning serotype to Salmonella enterica isolated from poultry environmental samples. - (Abstract Only)
Lungu, B., Sanchez-Ingunza, R., Guard, J.Y. 2013. Evaluation of a dkgB linked intergenic sequence ribotyping (ISR) method for assigning serotype to Salmonella enterica isolated from poultry environmental samples. American Association of Avian Pathologists. p. 13.
Serotypes of Salmonella enterica Present in the Internal Organs of Mice Caught On-farm From 1995 - 1998. - (Abstract Only)
Guard, J.Y., Stewart, T.E., Sanchez-Ingunza, R. 2012. Serotypes of Salmonella enterica Present in the Internal Organs of Mice Caught On-farm From 1995 - 1998. Poultry Science Association Meeting Abstract. p 291.
Impact of Strain Variation on the Ability of Biosensor Technology to Detect Salmonella enterica - (Abstract Only)
Guard, J.Y. 2012. Impact of Strain Variation on the Ability of Biosensor Technology to Detect Salmonella enterica. International Association for Food Protection. p. 1-45.
Use of Intragenic Sequence Ribotyping (ISR) for serotyping Salmonella obtained from poultry and their environment - (Abstract Only)
Pulido-Landinez, M., Sanchez-Ingunza, R., Guard, J.Y., Pinheiro Nascimento, V. 2012. Use of Intragenic Sequence Ribotyping (ISR) for serotyping Salmonella obtained from poultry and their environment. American Association of Avian Pathologists. p. 17.
Differential Transcriptional Regulation Between Egg-contaminating and Non-egg-contaminating Strains of Salmonella Enteritidis - (Abstract Only)
Orfe, L., Desai, P.T., Shah, D., Call, D.R., Guard, J.Y., Shah, D.H. 2012. Differential Transcriptional Regulation Between Egg-contaminating and Non-egg-contaminating Strains of Salmonella Enteritidis. American Society for Microbiology. p. B-2739.
Visible/NIR spectroscopy for Identifying Salmonella Infected Broilers - (Proceedings)
Accepted Publication (15-Aug-11)
Intergenic Sequence Ribotyping using a region neighboring dkgB links genovar to Kauffman-White serotype of Salmonella enterica - (Other)
Guard, J.Y. 2011. Intergenic Sequence Ribotyping using a region neighboring dkgB links genovar to Kauffman-White serotype of Salmonella enterica. National Center for Biotechnology Information (NCBI).
Hypothesis: A role for the mouse as an amplifier of Salmonella enterica on-farm - (Proceedings)
Guard, J.Y., Sanchez-Ingunza, R., Ahmer, B., Mcclelland, M., Henzler, D. 2011. Hypothesis: A role for the mouse as an amplifier of Salmonella enterica on-farm. Poultry Science Association, July 16, 2011, St. Louis, Mo. p.24.
Improving Food Safety by Understanding the Evolution of Egg-contaminating Salmonella Enteritidis - (Proceedings)
Guard, J.Y. 2011. Improving Food Safety by Understanding the Evolution of Egg-contaminating Salmonella Enteritidis. In: Proceedings of XXX International Seminar of AMEVEA. April 6-8, 2011, Bogota, Columbia. 2011CDROM.
Food Safety Categories:Contaminants and Contamination
Epidemiology
Pathogen Biology
Return to Search Results