1. Using population genetics, track bacterial migration and adaptation of foodborne pathogens through poultry processing and the associated environment. Evaluate the variations and influence of genetic and strain diversity from animal through the processing plant.
2. Examine the role of protozoa and other potential biological populations in the microbial ecology of foodborne pathogens through poultry processing.
3. Evaluate the potential for protozoa and other biological controls to be used as intervention or mitigation strategies for human pathogens in poultry processing and processing facilities.
4. Based on objectives 1-3, develop and evaluate physical and chemical intervention strategies to reduce contamination by foodborne pathogens of poultry products.
Approach: The focus of this research would be called the 'transmission phase' by epidemiologists or the 'migration phase' by ecologists. Processing of poultry products creates many severe barriers to transmission such that most of the pathogens are lost. However, it is clear that the barriers are incomplete and enough pathogens survive and pass to human consumers to cause foodborne disease. It is reasonable to assume that bacteria have adaptive strategies that improve the chances that some clones will survive processing making transmission to humans possible. The objectives of this project are designed to determine the relative ability of genetically different clones of foodborne pathogens to survive barriers that are encountered in the poultry processing plant. This will be followed by studying specific biological barriers that are common to ecosystems and are often responsible for limiting migration of bacteria. It is also likely that protozoa will be found in the processing environment that are not only ineffective in killing pathogens but may even be protective. Therefore, we plan to study the mechanisms of destruction or protection as they are uncovered. The knowledge that is gained from these studies will be used to design enhanced barriers in an attempt to improve the microbiological benefits of poultry processing.
Sampling by sponge wipe or skin excision for recovery of inoculated Salmonella and Campylobacter from defeathered broiler carcasses
Berrang ME, Cox NA, Oakley BB.
J Food Prot. 2014 May;77(5):824-7.
Novel epidemic clones of Listeria monocytogenes, United States, 2011
Lomonaco S, Verghese B, Gerner-Smidt P, Tarr C, Gladney L, Joseph L, Katz L, Turnek M, Frace M, Chen Y, Brown E, Meinersmann RJ, Berrang ME, Knabel S
Emerg Infect Dis. 2014 Jan;19(1):147-50.
Salmonella and Campylobacter prevalence and concentration on pasture-raised broilers processed on-farm, in a mobile processing unit, and at small USDA-inspected facilities
Trimble LM, Alali WQ, Gibson K, Ricke SC, Crandall P, Jaroni D, Berrang ME
Food Control. 2013 Nov;34(1):177-82.
Use of germicidal ultra-violet light to reduce low numbers of Listeria monocytogenes on raw chicken meat
Berrang ME, Meinersmann RJ, Frank JF
J Food Prot. 2013 Nov;76(11):1969-71.
Proposed Model for the High Rate of Rearrangement and Rapid Migration Observed in Some IncA/C Plasmid Lineages
Meinersmann RJ, Lindsey RL, Bono JL, Smith TP, Oakley BB.
Appl Environ Microbiol. 2013 Aug;79(16):4806-14.
Contamination of raw poultry meat by airborne Listeria originating from a floor drain
Berrang ME, Frank JF, Meinersmann RJ.
J Appl Poult Res. 2013 Mar;22(1):132-6.
Campylobacter spp. recovered from the Upper Oconee River Watershed, Georgia in a 4-year study
Meinersmann RJ, Berrang ME, Little E.
Microb Ecol. 2013 Jan;65(1):22-7.
Generation of airborne Listeria innocua from model floor drains
Berrang ME, Frank JF.
J Food Prot. 2012 Jul;75(7):1328-31.
Controlling attachment and growth of Listeria monocytogenes in PVC model floor drains using a peroxide chemical, chitosan/arginine or heat - (Abstract Only)
Antimicrobial Effect of An Essential Oil Blend on Surface-attached Salmonella on Polyvinyl Chloride - (Abstract Only)
Chemical additive to maximize antimicrobial effect of chlorine during pilot scale immersion chilling of broiler carcasses - (Abstract Only)
Listeria monocytogenes from slaughter plant to fully cooked product - sources, sites and potential for intervention - (Abstract Only)
Berrang, M.E. 2013. Listeria monocytogenes from slaughter plant to fully cooked product - sources, sites and potential for intervention. International Poultry Scientific Forum. P.18.
A chemical additive to limit potential bacterial contamination in chill tanks - (Abstract Only)
Schambach, B.T., Berrang, M.E., Harrison, M. 2012. A chemical additive to limit potential bacterial contamination in chill tanks [abstract]. International Poultry Scientific Forum. p. 3.
Chill water additive controls transfer of Salmonella and Campylobacter by improved chlorine efficacy - (Abstract Only)
Accepted Publication (17-Oct-12)
Germicidal ultra-violet light to eliminate low numbers of Listeria monocytogenes on raw chicken meat - (Abstract Only)
Accepted Publication (17-Oct-12)
Listeria monocytogenes in broiler processing â€“ sources, sites and solutions - (Proceedings)
Berrang, M.E. 2012. Listeria monocytogenes in broiler processing â€“ sources, sites and solutions. XIII International Seminar on Poultry Production and Pathology. November 21-23, 2012. Vina del Mar, Chile.
Contamination of raw poultry meat by airborne listeria originating from a floor drain - (Abstract Only)
Berrang, M.E., Frank, J.F., Meinersmann, R.J. 2012. Contamination of raw poultry meat by airborne listeria originating from a floor drain [abstract]. Poultry Science. 91(Suppl.1):156-157.
A chemical additive to limit transfer of salmonella and campylobacter during immersion chill - (Abstract Only)
Schambach, B.T., Berrang, M.E., Harrison, M.A. 2012. A chemical additive to limit transfer of salmonella and campylobacter during immersion chill [abstract]. Poultry Science. 91(Suppl 1):78.
Experimental and applied approaches to control Salmonella in broiler processing - (Popular Publication)
Berrang, M.E. 2012. Experimental and applied approaches to control Salmonella in broiler processing. The Poultry Informed Professional. 123:1-3.
Recent results on pathogen intervention during poultry processing - (Abstract Only)
Berrang, M.E. 2012. Recent results on pathogen intervention during poultry processing. ARS Food Safety and Inspection Service Research Workshop. February 17-20,2012. Shepardstown, WV.
Generation of airborne listeria from floor drain - (Abstract Only)
Berrang, M.E., Frank, J. 2012. Generation of airborne listeria from floor drain [abstract]. 2012 International Poultry Scientific Forum, January 23-24, 2012, Atlanta, Georgia. p.4.
Control of salmonella at the chill tank - (Abstract Only)
Berrang, M.E. 2011. Control of salmonella at the chill tank. National Meeting on Poultry Health and Processing sponsored by Delmarva Poultry Industry, Inc. October 12-13,2011. Ocean City, Maryland.
Campylobacter Recovered from the Upper Oconee River Basin, Georgia, in a four-year study - (Abstract Only)
Meinersmann, R.J., Berrang, M.E. 2011. Campylobacter Recovered from the Upper Oconee River Basin, Georgia, in a four-year study. Campylobacter Helicobacter and Related Organisms International Workshop. August 28-September 1, 2011. Vancouver, BC, Canada.