Food Safety Research Information Office
Title:Molecular Approaches for the Identification and Characterization of Antimicrobial Resistance in Foodborne Pathogens
Objective:

1. Identify and characterize potential genetic markers within and across genera of the high priority foodborne organisms for poultry attribution. With current priorities, the organisms should include Salmonella and Campylobacter.

2. Determine unique characteristics of high priority serotypes of antimicrobial resistant foodborne bacteria and those of highly resistant or multi-resistant genotypes with novel phenotypes.

3. Evaluate the role of innovative chemical and/or biological treatments, such as arsenicals, prebiotics, or ammonium compounds and how they affect the prevalence and type of resistant pathogens or resistance genes.

More Info:
Approach:
In the previous project plan, studies were initiated in order to provide a basic understanding of the development, prevalence, dissemination, and persistence of antimicrobial resistance. Molecular methods were developed for rapid identification of bacterial species and antimicrobial resistance genes. The goal of the proposed project plan is to characterize antimicrobial resistant foodborne pathogens and commensals from the extensive National Antimicrobial Resistance Monitoring System (NARMS) bacterial culture collection and other foodborne bacterial culture collections using molecular tools. For Objective 1, genomic sequencing will be performed initially on bacterial isolates from the NARMS program to identify potential genes which can be used as genetic markers. Genetic markers will differentiate bacteria from poultry from the other major food animal sources. This information is critical for tracing bacterial sources in foodborne outbreaks or contamination of environmental areas. For
Objective 2, unique characteristics of high priority serotypes or subtypes of antimicrobial resistant foodborne bacteria will be examined in detail using genomic sequencing, microarray analysis, and PCR. Isolates that exhibit high levels of resistance, multi-drug resistant genotypes or novel phenotypes will be given priority. Vehicles for the dissemination of resistance genes (e.g. plasmids, transposons, and integrons) will be given special focus. We will detect new or emerging antimicrobial resistance in foodborne bacteria which is essential for understanding development of antimicrobial resistance.
In Objective 3, target bacterial populations identified from the above objectives will be tested for resistance to non-antimicrobial chemicals or solutions (biocides) commonly used in poultry or poultry processing. Resistance levels of isolates will be assessed followed by genetic analysis of the genes encoding the resistance. This will include bacterial conjugation to determine if the genes may be transferred within and between bacteria as well as cloning and characterization of the resistance genes. These validation studies will provide important data on resistance to commercially based non-antimicrobials in poultry processing.
Funding Source:United States Department of Agriculture (USDA), Agricultural Research Service (ARS)
Type:Appropriated
Start Date:2011
End Date:2016
Project Number:6612-32000-006-00
Accession Number:422276
Institutions:USDA/ARS - South Atlantic Area
Investigators:Englen, Mark
Frye, Jonathan
Jackson, Charlene
Project Reports:2013 Annual Report
2012 Annual Report
Published Journal
Articles USDA
ARS (NP 108):
Antimicrobial resistance genes in multidrug-resistant Salmonella enterica isolated from animals, retail meats, and humans in the United States and Canada
Glenn LM, Lindsey RL, Folster JP, Pecic G, Boerlin P, Gilmour MW, Harbottle H, Zhao S, McDermott PF, Fedorka-Cray PJ, Frye JG.
Microb Drug Resist. 2013 Jun;19(3):175-84.
Human-associated methicillin-resistant Staphylococcus aureus from a subtropical recreational marine beach
Plano LR, Shibata T, Garza AC, Kish J, Fleisher J, Sinigalliano CD, Gidley ML, Withum K, Elmir SM, Hower S, Jackson CR, Barrett JB, Cleary T, Davidson M, Davis J, Mukherjee S, Fleming LE, Solo-Gabriele HM .
Microb Ecol. 2013 May;65(4):1039-51.
Clonally related methicillin-resistant Staphylococcus aureus isolated from short-finned pilot whales (Globicephala macrorhynchus), human volunteers, and a bayfront cetacean rehabilitation facility
Hower S, Phillips MC, Brodsky M, Dameron A, Tamargo MA, Salazar NC, Jackson CR, Barrett JB, Davidson M, Davis J, Mukherjee S, Ewing RY, Gidley ML, Sinigalliano CD, Johns L, Johnson FE 3rd, Adebanjo O, Plano LR.
Microb Ecol. 2013 May;65(4):1024-38.
Prevalence and characterization of methicillin-resistant Staphylococcus aureus isolates from retail meat and humans in Georgia
Jackson CR, Davis JA, Barrett JB.
J Clin Microbiol. 2013 Apr;51(4):1199-207.
Pathogenicity of dodecyltrimethylammonium chloride-resistant Salmonella enterica
Kautz MJ, Dvorzhinskiy A, Frye JG, Stevenson N, Herson DS.
Appl Environ Microbiol. 2013 Apr;79(7):2371-6.
The intestinal fatty acid propionate inhibits Salmonella invasion through the post-translational control of HilD
Hung CC, Garner CD, Slauch JM, Dwyer ZW, Lawhon SD, Frye JG, McClelland M, Ahmer BM, Altier C.
Mol Microbiol. 2013 Mar;87(5):1045-60.
Epidemiology and genotypic characteristics of methicillin-resistant Staphylococcus aureus strains of porcine origin
Molla B, Byrne M, Abley M, Mathews J, Jackson CR, Fedorka-Cray P, Sreevatsan S, Wang P, Gebreyes WA.
J Clin Microbiol. 2012 Nov;50(11):3687-93.
Analysis of antimicrobial resistance genes detected in multiple-drug-resistant Escherichia coli isolates from broiler chicken carcasses
Glenn LM, Englen MD, Lindsey RL, Frank JF, Turpin JE, Berrang ME, Meinersmann RJ, Fedorka-Cray PJ, Frye JG.
Microb Drug Resist. 2012 Aug;18(4):453-63.
Characteristics of plasmids in multi-drug-resistant Enterobacteriaceae isolated during prospective surveillance of a newly opened hospital in Iraq
Huang XZ, Frye JG, Chahine MA, Glenn LM, Ake JA, Su W, Nikolich MP, Lesho EP.
PLoS One. 2012;7(7):1-8.
Non-Journal Publications:
Genetic Mechanisms of antimicrobial resistance identified in Salmonella enterica, Escherichia coli, and Enteroccocus spp. isolated from U.S. food animals - (Review Article)
Frye, J.G., Jackson, C.R. 2013. Genetic Mechanisms of antimicrobial resistance identified in Salmonella enterica, Escherichia coli, and Enteroccocus spp. isolated from U.S. food animals. Frontiers in Microbiology. 4(135):1-22.
Analysis of antimicrobial resistance mechanisms in MDR bacteria by microarray and high-throughput sequencing - (Abstract Only)
Frye, J.G. 2012. Analysis of antimicrobial resistance mechanisms in MDR bacteria by microarray and high-throughput sequencing. International Conference of the Korean Society of Veterinary Science. November 1-2, 2012. Seoul, Republic of Korea.
Microarray analysis of Salmonella Enteritidis Phage Type 8 treated with subinhibitory concentrations of trans-cinnamaldehyde or eugenol - (Abstract Only)
Kollanoor-Johny, A., Frye, J.G., Porwolik, S., Darre, M.J., Donoghue, A.M., Donoghue, D.J., Mcclelland, M., Venkitanarayanan, K. 2012. Microarray analysis of Salmonella Enteritidis Phage Type 8 treated with subinhibitory concentrations of trans-cinnamaldehyde or eugenol [abstract]. Poultry Science. 91(Suppl.1):76-77.
Analysis and comparison of antimicrobial resistance mechanisms and plasmids found in multi-drug resistant (MDR) Salmonella enterica and Escherichia coli - (Abstract Only)
Frye, J.G., Hiott, L.M., Barrett, J.B., Glenn, L.M., Englen, M.D., Jackson, C.R. 2012. Analysis and comparison of antimicrobial resistance mechanisms and plasmids found in multi-drug resistant (MDR) Salmonella enterica and Escherichia coli. American Society for Microbiology 112th General Meeting, June 16-19, 2012, San Francisco, California. p. 143.
Application of multiplex PCR, pulsed-field gel electrophoresis (PFGE), and BOX-PCR for molecular analysis of enterococci - (Book / Chapter)
Jackson, C.R., Spicer, L.M., Barrett, J.B., and Hiott, L.M. 2012. Application of multiplex PCR, Pulsed-Field Gel Electrophoresis (PFGE), and BOX-PCR for molecular analysis of enterococci. In: Magdelden, S., editors. Gel Electrophoresis. Rijeka, Croatia: InTech-Open Access. p. 269-298.
Analysis of antimicrobial resistance mechanisms in multi-drug resistant (MDR) Salmonella enterica by high-throughput DNA sequencing - (Abstract Only)
Frye, J.G., Hiott, L.M., Barrett, J.B., Glenn, L.M., Englen, M.D., Jackson, C.R., Cray, P.J. 2012. Analysis of antimicrobial resistance mechanisms in multi-drug resistant (MDR) Salmonella enterica by high-throughput DNA sequencing. International Conference on Emerging Infectious Diseases, March 11-14, 2012, Atlanta, Georgia. p.180.
Food Safety Categories:Pathogen Biology
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