|Title:||Pre-Harvest Interventions for Application During Poultry Production to Reduce Food-Borne Bacterial Pathogens|
1. Develop and evaluate current and novel strategies to reduce food-borne pathogens in the poultry production environment. 1.a. Generate and apply chicken egg-yolk antibodies to reduce Salmonella and Campylobacter populations in broiler chickens. 1.b. Enhance the mucus-binding ability of Lactobacillus reuteri and L. salivarius cultures by growing them in mucin-containing media and compare the ability of enhanced and original cultures to reduce colonization of Salmonella and Campylobacter isolates in vivo. 1.c. Investigate the effects of Bacillus subtilis and Lactobacillus reuteri delivered in feed for reduction of Salmonella and Campylobacter colonization in the broiler chicken's gastrointestinal system.
2. Develop and evaluate potential alternatives to antimicrobials and other interventions in the control of food-borne pathogens (specifically bacteriophage and bacteriophage lytic enzymes, bacteriocins). 2.a. Identify and clone bacteriophage and prophage lytic enzymes using genomics analyses. 2.b. Express lytic enzymes, bacteriocins and holins in yeast.
Novel alternatives to traditional antibiotics are urgently needed for food-animal production. The approaches of this project are to 1) evaluate novel biocontrol strategies to reduce bacterial pathogen GI tract colonization of chickens, and 2) identify and characterize the biophysical properties of anti-bacterial peptides and lytic enzymes. Our approach includes application of specific egg-derived immunoglobulin, enhancing probiotic lactobacilli and determining synergism of probiotic treatments. This will be accompanied by isolation and assay of enzymes capable of lysing food-borne pathogens. The lytic enzymes and previously described bacteriocins will also be cloned for enhanced expression in yeast which can be readily incorporated into chicken feed. In vitro bacterial growth inhibition and in vivo chicken trials will be used to determine practical intervention approaches applicable to the poultry industry. By providing novel alternatives to antibiotic usage in poultry, the overall impact of this research will be a reduction in bacterial pathogens associated with chickens.
|Funding Source:||United States Department of Agriculture (USDA), Agricultural Research Service (ARS)|
|Institutions:||USDA/ARS - South Atlantic Area|
|Project Reports:||2013 Annual Report|
2012 Annual Report
ARS (NP 108):
Characterization and antigenicity of recombinant Campylobacter jejuni flagellar capping
Yeh HY, Hiett KL, Line JE, Seal BS.
J Med Microbiol. 2014 Apr;63(4):602-9.
Characterization and reactivity of broiler chicken sera to selected recombinant Campylobacter jejuni chemotactic proteins
Yeh HY, Hiett KL, Line JE, Seal BS.
Arch Microbiol. 2014 Mar 9. [Epub ahead of print]
Expression of a Clostridium perfringens genome-encoded putative N-acetylmuramoyl-L-alanine amidase as a potential antimicrobial to control the bacterium
Tillman GE, Simmons M, Garrish JK, Seal BS.
Arch Microbiol. 2013 Nov;195(10):675-81.
Prospecting environmental mycobacteria: combined molecular approaches reveal unprecedented diversity
Pontiroli A, Khera TT, Oakley B, Mason S, Dowd SE, Erenso G, Aseffa A, Courtenay O, Wellington EH .
PLoS One. 2013 Jul 18;8(7):e68648.
Alternatives to antibiotics: a symposium on the challenges and solutions for animal production
Seal BS, Lillehoj HS, Donovan DM, Gay CG.
Anim Health Res Rev. 2013 Jun;14(1):78-87.
Construction, expression, purification and antigenicity of recombinant Campylobacter jejuni flagellar proteins
Yeh HY, Hiett KL, Line JE, Oakley BB, Seal BS.
Microbiol Res. 2013 May 6;168(4):192-8.
Characterization of bacteriophages virulent for Clostridium perfringens and identification of phage lytic enzymes as alternatives to antibiotics for potential control of the bacterium
Poult Sci. 2013 Feb;92(2):526-33.
Complete genome sequence of the podoviral bacteriophage ÷CP24R, which is virulent for Clostridium perfringens
Morales CA, Oakley BB, Garrish JK, Siragusa GR, Ard MB, Seal BS.
Arch Virol. 2012 Apr;157(4):769-72.
Molecular characterization of podoviral bacteriophages virulent for Clostridium perfringens and their comparison with members of the Picovirinae
Volozhantsev NV, Oakley BB, Morales CA, Verevkin VV, Bannov VA, Krasilnikova VM, Popova AV, Zhilenkov EL, Garrish JK, Schegg KM, Woolsey R, Quilici DR, Line JE, Hiett KL, Siragusa GR, Svetoch EA, Seal BS.
PLoS One. 2012;7(5):e38283.
Development and stability of bacteriocin resistance in Campylobacter spp
Hoang KV, Stern NJ, Lin J.
J Appl Microbiol. 2011 Dec;111(6):1544-50.
Characterization of Bacteriophages Virulent for Clostridium perfringens and Identification of Phage Lytic Enzymes as Alternatives to Antibiotics for Potential Control of the Bacterium - (Proceedings)
Seal, B.S. 2013. Characterization of Bacteriophages Virulent for Clostridium perfringens and Identification of Phage Lytic Enzymes as Alternatives to Antibiotics for Potential Control of the Bacterium. United States Animal Health Association Proceedings. 116:577-583.
Expression and Characterization of Recombinant Campylobacter jejuni Chemotactic Proteins - (Abstract Only)
Accepted Publication (21-Feb-13)
Bacteriocins and Bacteriophage Lytic Proteins from Russian Federation and USA Collaborations to Control Antibiotic Resistant Bacterial Pathogens - (Abstract Only)
Accepted Publication (16-Oct-12)
Characterization of Bacteriophages Virulent for Clostridium perfringens and Identification of Phage Lytic Enzymes as Alternatives to Antibiotics for Potential Control of the Bacterium. - (Abstract Only)
Accepted Publication (03-Oct-12)
Construction, Expression, and Characterization of Flagellar Proteins for the Food-borne Pathogen Campylobacter jejuni - (Abstract Only)
Accepted Publication (19-Jun-12)
Characterization of bacteriophages virulent for Clostridium perfringens and identification of phage lytic enzymes as alternatives to antibiotics for potential control of the bacterium - (Abstract Only)
Accepted Publication (17-May-12)
Bacteriocins and Bacteriophage Lytic Proteins as Alternatives to Antibiotics from Russian Federation and USA Collaborations - (Abstract Only)
Accepted Publication (01-Apr-12)
Molecular Characterization of Podoviridae Bacteriophages Virulent for Clostridium perfringens and Comparison of Their Predicted Lytic Proteins - (Abstract Only)
Accepted Publication (30-Dec-11)
Influence of Culture Methods on Recovery of Salmonella Serotypes - (Proceedings)
Cox Jr, N.A., Cason Jr, J.A., Cray, P.J. 2011. Influence of culture methods on recovery of Salmonella serotypes. Proceedings of the 46th National Meeting on Poultry Health and Processing. October 11-13, 2011, Ocean City, Maryland. p.119-122.
|Food Safety Categories:||Food and Feed Composition and Characteristics|
On-Farm Food Safety
|Farm-to-Table categories:||On-farm food production|
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