Food Safety Research Information Office
Title:Optical Detection of Food Safety and Food Defense Hazards
Objective:

1. Develop methods and instruments to identify food safety hazards throughout various stages of poultry and egg production and processing.

2. Detect and characterize foodborne pathogens, toxins, and bacterial threat agents with rapid optical methods.

3. Develop and evaluate detection methods for foodborne pathogens and toxins with nanotechnology.

More Info:
Approach: Various optical (imaging) methods will be used for detecting intentional and unintentional contaminants and bacterial pathogens of food products. A real-time in-line hyperspectral imaging system will be used to rapidly detect diseased and contaminated broiler carcasses in processing plants. A monochromatic imaging system will be used for detecting cracks in table and hatching eggs. Hyperspectral microscopic imaging system, Raman imaging instrument and Fourier transform infrared spectrometer will be used to detect biofilms and foreign materials on the surfaces of food processing equipment. Visible/near-infrared hyperspectral imaging systems will be used to rapidly detect and characterize foodborne pathogens associated with poultry products and bacterial threat agents. Nanotechnology will be used for detection of foodborne pathogens and toxins. Collaboration with ARS Environmental Microbial and Food Safety Laboratory, BARC, FSIS, AMS, and the University of Georgia Nano Science and Engineering Center will be used to enhance the research.
Funding Source:United States Department of Agriculture (USDA), Agricultural Research Service (ARS)
Type:Appropriated
Start Date:2011
End Date:2016
Project Number:6612-42000-041-00
Accession Number:421113
Institutions:USDA/ARS - South Atlantic Area
Investigators:Gamble, Gary
Lawrence, Kurt
Park, Bosoon
Windham, William
Yoon, Seung-Chul
Project Reports:2011 Annual Report
Published Journal
Articles USDA
ARS (NP 108):
Surface enhanced Raman scattering (SERS) with biopolymer encapsulated silver nanosubstrates for rapid detection of foodborne pathogens
Sundaram J, Park B, Kwon Y, Lawrence KC.
Int J Food Microbiol. 2013 Oct;167(1):67-73.
Stable silver/biopolymer hybrid plasmonic nanostructures for high performance surface enhanced Raman scattering (SERS)
Sundaram J, Park B, Kwon Y.
J Nanosci Nanotechnol. 2013 Aug;13(8):5382-90.
Detection by Hyperspectral Imaging of Shiga Toxin-Producing Escherichia coli Serogroups O26, O45, O103, O111, O121, and O145 on Rainbow Agar
Windham WR, Yoon SC, Ladely SR, Haley JA, Lawrence KC, Park B, Narang N, Cray Jr WC .
J Food Prot. 2013 Jul;76(7):112936.
Differentiation of big-six non-O157 shiga-toxin producing escherichia coli (STEC) on spread plates of mixed cultures using hyperspectral imaging
Yoon SC, Windham WR, Ladely SR, Heitschmidt GW, Lawrence KC, Park,B, Narang N, Cray Jr WC .
J Food Meas Charact. 2013 Jun;7(2):47-59.
Hyperspectral imaging for differentiating colonies of non-O157 shiga-toxin producing echerichia coli (STEC) serogroups on spread plates of pure cultures
Yoon SC, Windham WR, Ladely SR, Heitschmidt GW, Lawrence KC, Park B, Narang N, Cray Jr WC .
J Near Infrared Spectrosc. 2013 Mar;21(2):81-95.
Detection and differentiation of Salmonella serotypes using surface enhanced Raman scattering (SERS) technique
Sundaram J, Park B, Hinton A, Lawrence K, Kwon Y.
J Food Meas and Char. 2013 Mar;7(1):1-12.
Acousto-Optic Tunable Filter Hyperspectral Microscope Imaging Method for Characterizing Spectra from Foodborne Pathogens
Park B, Yoon SC, Lee S, Sundaram J, Windham W, Hinton A, Lawrence K.
Transactions of the ASABE. 2012 Dec;55(5):.
Influence of color on dielectric properties of marinated poultry breast meat
Samuel D, Trabelsi S.
Poult Sci. 2012 Aug;91(8):2011-6.
The effect of regions of interest and spectral pre-processing on the detection of non-0157 Shiga-toxin producing Escherichia coli serogroups on agar media by hyperspectral imaging
Windham W, Yoon SC, Ladley S, Heitschmidt G, Lawrence K, Park B, Narang N, William C .
J Near Infrared Spectrosc. 2012 Jun;20(5):547-58.
High-resolution single-molecule recognition imaging of the molecular details of ricin-aptamer interaction
Wang B, Guo C, Zhang M, Park B, Xu B.
J Phys Chem B. 2012 May 3;116(17):5316-22.
Following aptamer-ricin specific binding by single molecule recognition and force spectroscopy measurements
Wang B, Guo C, Chen G, Park B, Xu B.
Chem Commun (Camb). 2012 Feb 4;48(11):1644-6.
Classification and structural analysis of live and dead Salmonella cells using Fourier transform infrared spectroscopy and principal component analysis
Sundaram J, Park B, Hinton A Jr, Yoon SC, Windham WR, Lawrence KC.
J Agric Food Chem. 2012 Feb 1;60(4):991-1004.
Influence of growth rate on occurrences of pale muscle in broilers
Samuel DD, Billard L, Pringle D, Wicker L.
J Sci Food Agric. 2012 Jan 15;92(1):78-83.
The use of dielectric spectroscopy as a tool for predicting meat quality in poultry
Samuel D, Trabelsi S, Karnuah A, Anthony N, Aggrey S.
Int J Poult Sci. 2012;11(9):551-5.
Motion compensated image processing and optimal parameters for egg crack detection using modified pressure
Yoon SC, Lawrence K, Jones D, Heitschmidt G, Park B.
Sens and Instrumen Food Qual. 2011 Dec;5(5):172-84.
Non-journal publications:
Food toxin detection with atomic force microscope- (Book / Chapter)
Accepted Publication (19-Jul-13)
Nanocolloid substrate for surface enhanced Raman scattering sensor for biological applications - (Book / Chapter)
Accepted Publication (20-Jun-13)
Classification of gram-positive and gram-negative foodborne pathogenic bacteria with hyperspectral microscope imaging - (Proceedings)
Accepted Publication (13-Jun-13)
Development of classification models to detect salmonella enteritidis and salmonella typhimurium found in poultry carcass rinses by visible-near infrared hyperspectral imaging - (Proceedings)
Accepted Publication (07-May-13)
The use of silver nanorod array based surface enhanced Raman scattering sensor for food safety applications - (Book / Chapter)
Accepted Publication (08-Apr-13)
Hyperspectral imaging for detection of non-O157 shiga-toxin producing escherichia coli(STEC) serogroups on spread plates of mixed cultures - (Proceedings)
Yoon, S.C., Windham, W.R., Ladely, S.R., Heitschmidt, G.W., Lawrence, K.C., Park, B., Narang, N., Cray Jr, W.C. 2012. Hyperspectral imaging for detection of non-O157 shiga-toxin producing escherichia coli(STEC) serogroups on spread plates of mixed cultures. Proceedings of SPIE. 8369-09.
Classification of shiga toxin-producing escherichia coli (STEC) serotypes with hyperspectral microscope imagery - (Proceedings)
Accepted Publication (23-Apr-12)
Detection and characterization of foodborne pathogenic bacteria with hyperspectral microscope imaging - (Abstract Only)
Park, B., Sundaram, J., Hinton Jr, A., Yoon, S.C., Lawrence, K.C., Windham, W.R. 2012. Detection and characterization of foodborne pathogenic bacteria with hyperspectral microscope imaging. Agricultural Engineering International Conference. CIGR-AgEng2012 CD-ROM, No. C-0994.
Differentiating non-0157:H7 STEC serogroups from ground beef plated on agar media by hyperspetral imaging - (Abstract Only)
Accepted Publication (06-Mar-12)
Detection of toxins in single molecule level using deoxyribonucleic acid aptamers - (Abstract Only)
Wang, B., Park, B., Guo, C., Xu, B. 2012. Detection of toxins in single molecule level using deoxyribonucleic acid aptamers [abstract]. Biosensors World Congress. Biosensors 2012 CDROM, No. 0169.
Identification and characterization of salmonella serotypes using DNA spectral characteristics by fourier transform infrared (FT-IR) spectroscopy - (Proceedings)
Sundaram, J., Park, B., Hinton Jr, A., Yoon, S.C., Windham, W.R., Lawrence, K.C. 2012. Identification and characterization of salmonella serotypes using DNA spectral characteristics by fourier transform infrared (FT-IR) spectroscopy. Proceedings of SPIE. 8369. DOI:10.1117/12.918585.
Preparation and characterization of silver particle encapsulated biopolymer nano-colloid substrate for surface enhanced raman scattering (SERS) for biological applications - (Abstract Only)
Sundaram, J., Park, B., Lawrence, K.C., Yongkuk, K. 2012. Preparation and Characterization of silver particle encapsulated biopolymer nano-colloid substrate for surface enhanced raman scattering (SERS) for biological applications [abstract]. American Chemical Society. ACS meeting, March 25-29, Santiago, USA.
Hyperspectral imaging for identifying non-O157 shiga toxin-producing escherichia coli (STEC) serotypes - (Proceedings)
Accepted Publication (15-Aug-11)
Improved egg crack detection algorithm for modified pressure imaging system - (Proceedings)
Yoon, S.C., Lawrence, K.C., Jones, D.R., Heitschmidt, G.W., Park, B. 2011. Improved egg crack detection algorithm for modified pressure imaging system. SPIE. Vol. 8027:U1-7.
AOTF hyperspectral microscope imaging for foodborne pathogenic bacteria detection - (Proceedings)
Park, B., Lee, S., Yoon, S.C., Sundaram, J., Windham, W.R., Hinton Jr, A., Lawrence, K.C. 2011. AOTF hyperspectral microscope imaging for foodborne pathogenic bacteria detection. Proceedings of SPIE. 8027:1-11.
Differentiation of live and dead salmonella cells using fourier transform infrared (FTIR) spectroscopy and principle component analysis (PCA) technique - (Abstract Only)
Sundaram, J., Park, B., Hinton Jr, A., Windham, W.R., Yoon, S.C., Lawrence, K.C. 2011. Differentiation of live and dead salmonella cells using fourier transform infrared (FTIR) spectroscopy and principle component analysis (PCA) technique [abstract]. American Society of Agricultural and Biological Engineers. ASABE meeting, August 3-7, Kentucky, USA.
Surface enhanced raman spectroscopy technique in rapid detection of live and dead salmonella cells - (Abstract Only)
Sundaram, J., Park, B., Zhao, Y., Hinton Jr, A., Windham, W.R., Yoon, S.C., Lawrence, K.C. 2011. Surface enhanced raman spectroscopy technique in rapid detection of live and dead salmonella cells [abstract]. American Chemistry Society Abstracts. 24th ACS National Meeting March 26-31, 2011, Anaheim, CA.
Near-infrared Hyperspectral Reflectance Imaging for Early Detection of Sour Skin Disease in Vidalia Sweet Onions - (Proceedings)
Wang, W., Li, C., Gitaitis, R., Tollner, E., Rains, G., Yoon, S.C. 2010. Near-infrared Hyperspectral Reflectance Imaging for Early Detection of Sour Skin Disease in Vidalia Sweet Onions. ASABE Annual International Meeting. Paper No. 1009106.
Detection of Sour Skin Diseases in Vidalia Sweet Onions using Near-Infrared Hyperspectral Imaging - (Proceedings)
Wang, W., Thai, C., Li, C., Gitaitis, R., Tollner, E., Yoon, S.C. 2009. Detection of Sour Skin Diseases in Vidalia Sweet Onions using Near-Infrared Hyperspectral Imaging. ASABE Annual International Meeting. Technical Paper 096364.
Partial least squares models for hyperspectral contaminant detection - (Proceedings)
Lawrence, K.C., Windham, W.R., Park, B. 2007. Partial least squares models for hyperspectral contaminant detection. Proc. 12th International Conf. Near Infrared Spectroscopy. p.595-603.
Multipurpose hyperspectral imaging system - (Research Technical Update)
Mao, C., Smith, D., Lanoue, M.A., Poole, G.H., Heitschmidt, G.W., Martinez, L., Windham, W.R., Lawrence, K.C., Park, B. 2005. Multipurpose hyperspectral imaging system. NASA Tech Briefs. 29(8):30-31 (Technical Article).
LED lighting for use in multispectral and hyperspectral imaging - (Proceedings)
Lawrence, K.C., Park, B., Windham, W.R., Heitschmidt, G.W. 2005. LED lighting for use in multispectral and hyperspectral imaging. ASABE Annual International Meeting. Paper No. 053073.
Comparison between visible/ NIR spectroscopy and hyperspectral imaging for detecting surface contaminants on poultry carcasses - (Proceedings)
Lawrence, K.C., Windham, W.R., Park, B., Smith, D., Poole, G.H. 2004. Comparison between visible/ NIR spectroscopy and hyperspectral imaging for detecting surface contaminants on poultry carcasses. Proceedings of SPIE. 5271.
Automated on-line fecal detection - digital eye guards against fecal contamination - (Review Article)
Windham, W.R., Lawrence, K.C., Park, B., Smith, D. 2004. Automated on-line fecal detection - digital eye guards against fecal contamination. WATT Poultry USA. 4(11):32-38.
Effect of broiler carcass washing on fecal contaminant imaging - (Proceedings)
Lawrence, K.C., Windham, W.R., Smith, D., Park, B. 2013. Effect of broiler carcass washing on fecal contaminant imaging. ASAE Paper No. 033122, Amer. Soc. Agric. Engrs., St. Joseph, MI.
Improving performance of real-time multispectral imaging system - (Proceedings)
Park, B., Yoon, S.C., Kise, M., Lawrence, K.C., Windham, W.R. 2008. Improving performance of real-time multispectral imaging system. ASABE Annual International Meeting. ASABE Paper No. 085024.
Food Safety Categories:Food and Feed Handling and Processing
Methodology
Food Defense and Security
Farm-to-Table categories:Food processing
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