Food Safety Research Information Office
Title:Metabolic Fate of Chemical and Biological Contaminants
Objective:

Objective 1. Investigate the kinetics of uptake, metabolism, distribution, and excretion of chemicals in food animals with the goal of reducing public exposure to chemical residues in food animal products (milk, meat, eggs).

Objective 2. Determine the fate of endogenous reproductive hormones, pharmaceuticals, and other chemicals in wastes of food animals, including transport through soil and water.

Objective 3. Develop sensitive and accurate analytical tools to rapidly detect and quantify chemicals identified in objectives 1 and 2.

More Info:
Approach: The broad objective of this project is to determine the fate of natural and man-made chemicals in food animals and in food animal systems (wastes, soil, water). Three broad classes of chemicals will be targeted for study: (1) veterinary drugs or feed additives administered to food animals under extra-label use conditions, (2) endogenous steroid hormones, and (3) novel developmental chemicals of potential utility to the livestock industry. Use of veterinary chemicals in an extra-label manner without knowledge of residue depletion kinetics has led to unsafe residues in meat products. Endogenous steroid hormones excreted by livestock are highly potent endocrine-disrupting compounds that are thought to disrupt the development of aquatic species after their entry into surface waters. Finally, chemical technologies developed by the ARS, e.g., chloroxyanions and nitro compounds, are active against Salmonella and E. coli pathogens in livestock immediately prior to slaughter, but the impacts of chemical residues in meat products have not been fully investigated for these compounds. Regardless of the chemical class being investigated, the development of sensitive and accurate analytical tools is critical completion of the objectives. Therefore, a significant portion of the project is devoted to developing the analytical tools required to ensure success of the project. The overall project goal is to understand the broad impact that chemical residues play in influencing food and environmental safety.
Funding Source:United States Department of Agriculture (USDA), Agricultural Research Service (ARS)
Type:Appropriated
Start Date:2011
End Date:2016
Project Number:5442-32000-014-00
Accession Number:421009
Institutions:USDA/ARS - Northern Plains Area
Investigators:Hakk, Heldur
Shappell, Nancy
Shelver, Weilin
Smith, David
Project Reports:2013 Annual Report
2012 Annual Report
2011 Annual Report
Published Journal
Articles USDA
ARS (NP 108):
Potential bioactivity and association of 17-estradiol with the dissolved and colloidal fractions of manure and soil
Chambers K, Casey F, Hakk H, Desutter T, Shappell N.
Sci Total Environ. 2014 Oct 1;494-5:58-64.
Development of a physiologically based pharmacokinetic model for flunixin in cattle (Bos taurus)
Leavens T, Tell L, Kissell L, Smith D, Wagner S, Shelver W, Huali W, Baynes R, Riviere J.
Food Addit Contam Part A. 2014 Aug 1. [Epub ahead of print]
Sorption and metabolism of 17-estradiol-17-sulfate in sterilized soil-water systems
Bai X, Casey FX, Hakk H, Desutter TM, Oduor PG, Khan E .
Chemosphere. 2014 Mar 12. [Epub ahead of print]
Fate of 17B-estradiol in anaerobic lagoon digesters
Hakk H, Sikora L, Casey FX, Larsen G .
J Environ Qual. 2014 Feb;43(2):701-8.
Effect of intravenous or oral sodium chlorate administration on the fecal shedding of Escherichia coli in sheep
Smith DJ, Taylor JB, West M, Herges G.
J Anim Sci. 2013 Dec;91(12):5962-9.
Dissipation and transformation of 17β-estradiol-17-sulfate in soil-water systems
Bai X, Casey FX, Hakk H, Desutter TM, Oduor PG, Khan E.
J Hazard Mater. 2013 Sep 15;260:733-9.
Egg wash wastewater: Estrogenic risk or environmental asset?
Shappell NW .
Integr Environ Assess Manag. 2013 Jul;9(3):517-23.
Bioassay of estrogenicity and chemical analysis of estrogens in streams across the United States associated with livestock operations
Alvarez DA, Shappell NW, Billey LO, Bermudez DS, Wilson VS, Kolpin DW, Perkins SD, Evans N, Foreman WT, Gray JL, Shipitalo MJ, Meyer MT .
Water Res. 2013 Jun 15;47(10):334763.
Comparison of ELISA and LC-MS/MS for the measurement of flunixin plasma concentrations in beef cattle after intravenous and subcutaneous administration
Shelver WL, Tell LA, Wagner S, Wetzlich SE, Baynes RE, Riviere JE, Smith DJ .
J Agric Food Chem. 2013 Mar 7;61(11):267986.
Radioassay-based approach to investigate fate and transformation of conjugated and free estrogens in an agricultural soil
Shrestha SL, Casey F, Hakk H, Padmanabham G.
Environ Eng Sci. 2013 Feb 14;30(2):89-96.
Toxicity and metabolism of nitroalkanes and substituted nitroalkanes
Smith DJ, Anderson RC .
J Agric Food Chem. 2013 Jan 30;61(4):763-79.
Strip-based immunoassay for the simultaneous detection of the neonicotinoid insecticides imidacloprid and thiamethoxam in agricultural products
Xu T, Xu QG, Li H, Wang J, Li QX, Shelver WL, Li J.
Talanta. 2012 Nov 15;101:85-90.
Invited review: Efficacy, metabolism, and toxic responses to chlorate salts in food and laboratory animals
Smith DJ, Oliver CE, Taylor JB, Anderson RC.
J Anim Sci. 2012 Nov;90(11):4098-117.
Fate and transformation of an estrogen conjugate and its metabolites in agricultural soils
Shrestha SL, Casey FX, Hakk H, Smith DJ, Padmanabhan G.
Environ Sci Technol. 2012 Oct 16;46(20):11047-53.
Kinetics and disposition of orally dosed sodium chlorate in sheep
Smith DJ, Taylor JB.
J Anim Sci. 2012 Jun;90(6):2026-34.
E-Screen evaluation of sugar beet feedstuffs in a case of reduced embryo transfer efficiencies in cattle: the role of phytoestrogens and zearalenone
Shappell NW, Mostrom MS, Lenneman EM.
In Vitro Cell Dev Biol Anim. 2012 Apr;48(4):216-28.
In vitro dissolution and in vivo absorption of calcium [1-14C]butyrate in free or protected forms
Smith DJ, Barri A, Herges G, Hahn J, Yersin AG, Jourdan A.
J Agric Food Chem. 2012 Mar 8;60(12):3151-7.
Water quality, sediment, and soil characteristics near Fargo-Moorhead urban areas as affected by major flooding of the Red River of the North
Guy AC, Desutter TM, Casey FX, Kolka R, Hakk H.
J Environ Qual. 2012 Mar;41(2):554-63.
Development of a UHPLC-MS/MS method for the measurement of chlortetracycline degradation in swine manure
Shelver WL, Varel VH.
Anal Bioanal Chem. 2012 Feb;402(5):1931-9.
Comparing biological effects and potencies of estrone and 17β-estradiol in mature fathead minnows, Pimephales promelas
Dammann AA, Shappell NW, Bartell SE, Schoenfuss HL.
Aquat Toxicol. 2011 Oct;105(3):559-68.
Non-Journal Publications:
Ractopamine uptake from soil by alfalfa (Medicago sativa) and wheat (Triticum aestivum)
(07-May-14)
Use of kidney inhibition swab test to evaluate antimicrobial residues in pork kidney from a market survey in Fargo, North Dakota - (Abstract Only)
(05-Mar-14)
Modeling sorption and degradation of 17B-estradiol-17-sulfate in agricultural soils - (Abstract Only)
Bai, X., Casey, F.X.M., Hakk, H., Shrestha, S.L., Desutter, T.M., Khan, E., Oduor, P.G. 2011. Modeling sorption and degradation of 17B-estradiol-17-sulfate in agricultural soils. American Geophysical Union. American Geophysical Union Annual Meeting, December 5-9, 2011, San Francisco, CA.
Comparison of ELISA and LC-MS/MS for the measurement of flunixin plasma concentrations in beef cattle after intravenous and subcutaneous administration - (Abstract Only)
Accepted Publication (02-Jul-13)
Application of kidney inhibition swab tests to evaluate penicillin-G residues in sow tissues and body fluids following intramuscular injection - (Abstract Only)
Accepted Publication (02-Jul-13)
Modulation of estrogenic exposure effects mediated through temperature and dietary regimens in male fat head minnows - (Abstract Only)
Accepted Publication (28-Jun-13)
E-Screen - potential tool for assessment of relative serum estrogenicity - (Abstract Only)
Accepted Publication (17-Mar-13)
Consumption of beef from cattle administered estrogenic growth promotants does not result in premature puberty and obesity using the swine model - (Proceedings)
Magolski, J.D., Anderson, G., Shappell, N.W., Vonnahme, K.A., Berg, E.P. 2012. Consumption of beef from cattle administered estogenic growth promotants does not result in premature puberty and obesity using the swine model. Proceedings 58th International Congress of Meat Science & Technology, August 12-17, 2012, Montreal, Canada. 58:D85.
Evaluation of surface waters associated with animal feeding operations for estrogenic chemicals and activity - (Abstract Only)
Shappell, N.W., Alvarez, D., Kolpin, D., Forman, W., Gray, J., Meyer, M. 2012. Evaluation of surface waters associated with animal feeding operations for estrogenic chemicals and activity. Society of Environmental Toxicology and Chemistry North American 33rd Annual Meeting, November 11-15, 2012, Long Beach, CA. p. 377.
Development of a UHPLC-MS/MS method for the measurement of chlortetracycline degradation in swine manure - (Abstract Only)
Shelver, W.L., Varel, V.H. 2012. Development of a UHPLC-MS/MS method for the measurement of chlortetracycline degradation in swine manure [absstract]. The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, March 11-15, 2012, Orlando, FL.
In vivo and in vitro estrogenicity and GC/MS/MS and LC/MS/MS quantification of estrogens in aqueous mixtures of raw and pelletized poultry litter - (Abstract Only)
Yonkos, L.T., Fisher, D.J., Friedel, E.A., Wilson, V.S., Hutchins, S.R., Lazorchak, J.M., Reddy, T.V., Shappell, N.W., Van Veld, P.A., Hale, R.C. 2011. In vivo and in vitro estrogenicity and GC/MS/MS and LC/MS/MS quantification of estrogens in aqueous mixtures of raw and pelletized poultry litter. Society of Environmental Toxicology and Chemistry North America 32nd Annual Meeting, November 13-17, 2011, Boston, MA. abstr. 17, p. 8.
Estrogenic activity – a frame of reference for some foods, agricultural wastewaters, and animal feedstuffs. - (Abstract Only)
Shappell, N.W. 2011. Estrogenic activity – a frame of reference for some foods, agricultural wastewaters, and animal feedstuffs. Society of Environmental Toxicology and Chemistry North America 32nd Annual Meeting, November 13-17, 2011, Boston, MA. Abstract 302. p. 70.
Food Safety Categories:On-Farm Food Safety
Contaminants and Contamination
Methodology
Farm-to-Table Categories:On-farm food production
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