Food Safety Research Information Office
Title:Protection of Food and Water Supplies from Pathogen Contamination

Determine the relationships between manure management and populations of human pathogens and antibiotic resistant bacteria (ARB) that result in new recommendations for best management practices (BMPs);

Develop effective methods and practices to protect crops from pathogen contamination;

Develop management practices to minimize the transport of pathogens (e.g. E. coli O157:H7, Cryptosporidium, enterococcus, Salmonella) from concentrated dairy and beef cattle operations to water resources.

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Approach: Conduct laboratory and field experiments to examine the important biological, chemical, and physical processes affecting the prevalence and distribution of pathogenic and antibiotic resistant bacteria on representative farms in the Santa Ana River watershed. Studies will be conducted at various scales to determine the persistence (survival) of E. coli O157:H7 in its sources on these farms and assess potential factors influencing pathogen survival in the root zone and contamination of leafy greens. Laboratory scale study will be conducted to quantify critical processes influencing the dissemination of pathogens in the watershed by runoff, streams and rivers. Factors influencing the treatment of contaminated surface waters by sand filtration will also be investigated to more fully assess its capabilities and potential weaknesses. Data obtained from these studies will be used to develop best management practices (BMPs) and low cost treatment technologies for immobilization and inactivation of pathogens from concentrated animal feeding operations (CAFOs) to water and food resources. Replaces 5310-32000-002-00D (1/11).
Funding Source:United States Department of Agriculture (USDA), Agricultural Research Service (ARS)
Start Date:2010
End Date:2015
Project Number:5310-32000-003-00
Accession Number:420973
Institutions:USDA/ARS - Pacific West Area
Investigators:Bradford, Scott
Ibekwe, Abasiofiok Mark
Project Reports:2011 Annual Report
Published Journal
Articles USDA
ARS (NP 108):
Physicochemical factors influencing the preferential transport of Escherichia coli in soils
Wang Y, Bradford SA, Simunek J.
Vadose Zone J. 2014 Jan 13;13(1):.
Potential human pathogenic bacteria in a mixed urban watershed as revealed by pyrosequencing
Ibekwe AM, Leddy M, Murinda SE.
PLoS One. 2013 Nov 20;8(11):e79490.
A theoretical analysis of colloid attachment and straining in chemically heterogeneous porous media
Bradford SA, Torkzaban S, Shapiro A.
Langmuir. 2013 Jun 11;29(23):6944-52.
Influence of bacterial communities based on 454-pyrosequencing on the survival of Escherichia coli O157:H7 in soils
Ma J, Ibekwe AM, Yang CH, Crowley DE.
FEMS Microbiol Ecol. 2013 Jun;84(3):542-54.
Sensitivity of the transport and retention of stabilized silver nanoparticles to physicochemical factors
Liang Y, Bradford SA, Simunek J, Vereecken H, Klumpp E.
Water Res. 2013 May 1;47(7):2572-82.
Transport and fate of microorganisms in soils with preferential flow under different solution chemistry conditions
Wang Y, Bradford S, Simunek J.
Water Resources Research. 2013 May;49(5):2424-36.
Colloid interaction energies for physically and chemically heterogeneous porous media
Bradford SA, Torkzaban S.
Langmuir. 2013 Mar 19;29(11):3668-76.
Transport and retention of multi-walled carbon nanotubes in saturated porous media: effects of input concentration and grain size
Kasel D, Bradford SA, Šimůnek J, Heggen M, Vereecken H, Klumpp E.
Water Res. 2013 Feb 1;47(2):933-44.
Assessing the diversity and composition of bacterial communities across a wetland, transition, upland gradient in Macon County Alabama
Shange R, Haugabrooks E, Ankumah R, Ibekwe A, Smith R, Dowd S.
Diversity. 2013;5(3):461-78.
Transport and fate of microbial pathogens in agricultural settings
Bradford SA, Morales VL, Zhang W, Harvey RW, Packman Ai, Mohanram A, Welty C.
Crit Reviews in Environ Sci Technol. 2013;43(8):775-893.
A framework for developing research protocols for evaluation of microbial hazards and controls during production that pertain to the quality of agricultural water contacting fresh produce that may be consumed raw
Harris LJ, Bender J, Bihn EA, Blessington T, Danyluk MD, Delaquis P, Goodridge L, Ibekwe AM, Ilic S, Kniel K, Lejeune JT, Schaffner DW, Stoeckel D, Suslow TV.
J Food Prot. 2012 Dec;75(12):2251-73.
Persistence of Escherichia coli O157:H7 in major leafy green producing soils
Ma J, Ibekwe AM, Crowley DE, Yang CH.
Environ Sci Technol. 2012 Nov 6;46(21):12154-61.
Colloid adhesive parameters for chemically heterogeneous porous media
Bradford SA, Torkzaban S.
Langmuir. 2012 Sep 25;28(38):13643-51.
Modeling colloid and microorganism transport and release with transients in solution ionic strength
Bradford S, Torkzaban S, Kim H, Simunek J.
Water Resour Res. 2012 Sep;48(9):W09509.
Causes and implications of colloid and microorganism retention hysteresis
Bradford SA, Kim H.
J Contam Hydrol. 2012 Sep;138-139:83-92.
Transport of ARS-labeled hydroxyapatite nanoparticles in saturated granular media is influenced by surface charge variability even in the presence of humic acid
Wang D, Bradford SA, Harvey RW, Hao X, Zhou D.
J Hazard Mater. 2012 Aug 30;229-230:170-6.
Impacts of bridging complexation on the transport of surface-modified nanoparticles in saturated sand
Torkzaban S, Wan J, Tokunaga TK, Bradford SA.
J Contam Hydrol. 2012 Aug;136-137:86-95.
Humic acid facilitates the transport of ARS-labeled hydroxyapatite nanoparticles in iron oxyhydroxide-coated sand
Wang D, Bradford SA, Harvey RW, Gao B, Cang L, Zhou D.
Environ Sci Technol. 2012 Mar 6;46(5):2738-45.
Facilitated transport of copper with hydroxyapatite nanoparticles in saturated sand
Wang D, Bradford S, Paradelo M, Peijnenburg W, Zhou D.
Soil Sci Soc Am J. 2012 Mar;76(2):375-88.
Bacterial community composition in low-flowing river water with different sources of pollutants
Ibekwe A, Leddy M, Bold R, Graves A.
FEMS Microbiol Ecol. 2012 Jan;79(1):155-66.
Distinct soil bacterial communities revealed under a diversely managed agroecosystem
Shange RS, Ankumah RO, Ibekwe AM, Zabawa R, Dowd SE.
PLos One. 2012;7(7):e40338.
Assimilable organic carbon (AOC) in soil water extracts using Vibrio harveyi BB721 and its implication for microbial biomass
Ma J, Ibekwe AM, Wang H, Xu J, Leddy M, Yang CH, Crowley DE.
PLoS One. 2012;7(4):e28519.
Microbiological evaluation of water quality from urban watersheds for domestic water supply improvement
Ibekwe AM, Murinda SE, Graves AK.
Int J Environ Res Public Health. 2011 Dec;8(12):4460-76.
Facilitated transport of Cu with hydroxyapatite nanoparticles in saturated sand: effects of solution ionic strength and composition
Wang D, Paradelo M, Bradford SA, Peijnenburg WJ, Chu L, Zhou D.
Water Res. 2011 Nov 15;45(18):5905-15.
Modeling colloid transport and retention in saturated porous media under unfavorable attachment conditions
Bradford S, Torkzaban S, Simunek J.
Water Resour Res. 2011 Oct;47(10):W10503.
Effects of fumigants on microbial diversity and persistence of E. coli O15:H7 in contrasting soil microcosms
Ibekwe AM, Ma J.
Sci Total Environ. 2011 Sep 1;409(19):3740-8.
Non-Journal Publications:
Metabolite profiling of foodborne disease significance – case study Escherichia coli O157 - (Abstract Only)
Transpositional inactivation of gadW enhances curli production and biofilm formation in Enterohemorrhagic Escherichia coli O157:H7 - (Abstract Only)
Comparative genomic and phenotypic characterization of super-shedder Escherichia coli O157:H7 - (Abstract Only)
Molecular insights into the unique phenotypes exhibited by super shed shiga toxin producing Escherichia coli O157 - (Abstract Only)
Proteins facilitating Escherichia coli O157 persistence at the bovine recto-anal junction (RAJ) squamous epithelial cells - (Abstract Only)
Food Safety Categories:On-Farm Food Safety
Contaminants and Contamination
Farm-to-Table Categories:On-farm food production
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