Food Safety Research Information Office
Title:Handling and Transport Stress Interactions with Pathogen Biology in Swine and Cattle
Objective:Objective 1: Identify physiological, immunological, endocrinological and gastrointestinal-microbiological alterations which occur in infected livestock when subjected to common managerial stressors.

Objective 2: Understand how handling and transportation stress influence livestock pathogens, such as, Salmonella and Campylobacter, which have the potential to detrimentally affect human health.

More Info:
Approach: We will subject livestock to both mixing and transportation and collect behavioral, physiologic, immunologic, endocrine, and bacterial data. The behavior data will include: agonistic encounters, loss of balance, vomiting, standing, lying, stereotypic behavior, and any other abnormal behaviors (i.e. shaking, jumping, etc). The physiologic, immunologic, and endocrine data will include: heart rate, body temperature, cortisol, epinephrine, norepinephrine, dopamine, immune cell populations, interferon-gamma, interleukin-1, interleukin-12, haptoglobin, alpha 1-acid glycoprotien, and immunoglobulins.

The bacterial data will include: DGGE pattern, total aerobes, anaerobes, Enterobacteriaceae counts, and the proportion of antimicrobial resistant Enterobacteriaceae in different compartments of the gastrointestinal tract and in mesenteric lymph nodes. Analysis of these collective data will allow for identification of key processes that create high pathogen loads at slaughter plants.

Our approach is a strategy that will 1) use a novel technique to monitor the progression of infection of stressed swine, 2) study the influence of mammalian stress hormones on bacteria, and 3) determine physiologically how a dietary supplement can impair bacterial infection. All three of these approaches will provide novel information on how stress influences bacterial pathogens.

Funding Source:United States Department of Agriculture (USDA), Agricultural Research Service (ARS)
Type:Appropriated
Start Date:2010
End Date:2013
Project Number:3602-32000-010-00
Accession Number:420795
Institutions:USDA/ARS - Midwest Area
Investigators:Eicher, Susan
Lay, Jr., Donald
Rostagno, Marcos
Project Reports:2013 Annual Report
2012 Annual Report
2011 Annual Report
Pending USDA ARS Publications (NP 108):
Published Journal
Articles USDA
ARS (NP 108):
Non-Journal Publications:
Group size alters postures, and maintenance, oral, locomotor and social behaviors of veal calves - (Abstract Only)
Abdelfattah, E.M., Schutz, M.M., Lay Jr, D.C., Marchant Forde, J.N., Eicher, S.D. 2013. Group size alters postures, and maintenance, oral, locomotor and social behaviors of veal calves. Journal of Animal Science. Proceedings.
Group size of veal calves does not affect production, physiological, or hematological indicators of welfare and has transient effects on health - (Abstract Only)
Abdelfattah, E.M., Schutz, M.M., Lay Jr, D.C., Marchant Forde, J.N., Eicher, S.D. 2013. Group size of veal calves does not affect production, physiological, or hematological indicators of welfare and has transient effects on health. Journal of Animal Science. Proceedings CD.
Dietary L-arginine supplementation modulates lipopolysaccharide-induced systemic inflammatory response in broiler chickens - (Abstract Only)
Tan, J.Z., Guo, Y.M., Eicher, S.D., Applegate, T.J. 2013. Dietary L-arginine supplementation modulates lipopolysaccharide-induced systemic inflammatory response in broiler chickens. Poultry Science Association Meeting Abstract. 92(E-suppl.1):.
Zinc’s impact on intestinal barrier function and zinc trafficking during coccidial caccine challenge - (Abstract Only)
Troche, C., Eicher, S.D., Applegate, T.J. 2012. Zinc’s impact on intestinal barrier function and zinc trafficking during coccidial caccine challenge. Poultry Science. 91(E-suppl.1):145.
Effect of stress on Salmonella, coliforms and lactobacilli in different portions of the intestinal tract of swine - (Abstract Only)
Rostagno, M.H., Richert, B.T., Lay Jr, D.C. 2012. Effect of stress on Salmonella, coliforms and lactobacilli in different portions of the intestinal tract of swine. Rowett Research Institute Institute National De La Resherche Agronomique Joint Symposium. Proceedings.
Effect of pre-slaughter stressors on intestinal microbial populations of pigs - (Abstract Only)
Rostagno, M.H., Richert, B.T., Lay Jr, D.C. 2012. Effect of pre-slaughter stressors on intestinal microbial populations of pigs. Joint Abstracts of the American Dairy Science and Society of Animal Science. Proceedings.
Cranberry juice and cranberry fiber are accepted by newly weaned pigs - (Abstract Only)
Accepted Publication (04-Apr-12)
Transportation and mixing with unfamiliar pigs increase the susceptibility of market-weight pigs to Salmonella enterica - (Abstract Only)
Rostagno, M.H., Lay Jr, D.C. 2012. Transportation and mixing with unfamiliar pigs increase the susceptibility of market-weight pigs to Salmonella enterica. Pig Veterinary Society International Congress Proceedings. Meeting Proceedings.
Salmonella serovars differentially stimulate bovine leukocyte responses in vitro - (Abstract Only)
Pan, D., Rostagno, M.H., Ebner, P., Eicher, S.D. 2011. Salmonella serovars differentially stimulate bovine leukocyte responses in vitro. Research Workers in Animal Diseases Conference Proceedings. 74P.
Dam heat load affects neonatal calves’ bacterial prevalence and innate immunity - (Abstract Only)
Pan, D., Lee, C.N., Rostagno, M.H., Eicher, S.D. 2011. Dam heat load affects neonatal calves’ bacterial prevalence and innate immunity. Journal of Dairy Science. 94 (E-Suppl. 1):744.
Food Safety Categories:On-Farm Food Safety
Pathogen Biology
Farm-to-Table Categories:On-farm food production
Food transport
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