Food Safety Research Information Office
Title:Pathogen Reduction and Processing Parameters in Poultry Processing Systems
Objective:

1. Develop, evaluate, and validate alternatives to current antimicrobials and sanitizers for processing poultry carcasses that meet national and international food safety standards. Specifically, alternatives to chlorine and chlorine dioxide, such as GRAS surfactants, ozone, acidified sodium chloride, and peroxyacids should be examined.

2. Characterize and evaluate poultry processing conditions associated with processed broiler carcasses that are heavily contaminated by Salmonella. This objective will include determining microbial recovery/extraction rates for poultry rinse sampling and examining survival characteristics for Salmonella serotypes that survive processing operations. Sampling should include whole carcasses that are high shedders, and mechanically-separated poultry parts.

3. Conduct studies on extrinsic factors that influence the survival and attachment of pathogenic, spoilage, and indicator microorganisms recovered from commercial poultry processing operations. These include water activity, pH, ozone, metal chelators, and organic acids.

4. Collaborate with the QSARU at Athens to develop emerging technologies for process control and correlate molecular and cultural analysis of microbial communities in poultry with process control and pathogen contamination.

More Info:
Approach: Technologies will be developed that can be utilized by commercial processors to reduce microbial contamination associated with poultry processing. Formulations for novel sanitizers will be developed, and improved techniques utilizing sanitizers already approved for commercial use in processing will be designed. Surfactant based sanitizers used alone or in combination with non-chlorine based sanitizers will be examined as alternatives to chlorine and chlorine dioxide for decreasing microbial contamination of poultry. Additionally, poultry processing conditions associated with broiler carcasses heavily contaminated by Salmonella will be evaluated and characterized. Cross contamination during processing will be examined by studying the role of these heavily contaminated carcasses in the spread of Salmonella during processing. Furthermore, factors that influence survival and attachment of pathogenic, spoilage, and indicator microorganisms on poultry skin will be examined. Microorganisms on poultry skin will be examined utilizing Benchtop scanning electron microscopy (SEM) with SEM software and with standard microbiological methods. Finally, in collaboration with the Quality Assessment Research Unit (QARU), correlations between molecular and cultural analysis of microbial communities on poultry skin will be examined and emerging technologies for process control will be developed. Technology will be designed for use in the rapid detection and identification of pathogenic bacteria associated with poultry processing and for the characterization of microbial biofilms on broiler skin. All research goals will be achieved through an interdisciplinary team approach utilizing knowledge and skills of Unit scientists and other scientists who possess unique skills and resources that will aid in the successful completion of this project.
Funding Source:United States Department of Agriculture (USDA), Agricultural Research Service (ARS)
Type:Appropriated
Start Date:2011
End Date:2016
Project Number:6612-41420-017-00
Accession Number:421037
Institutions:USDA/ARS - South Atlantic Area
Investigators:Hinton, Jr., Arthur
Rothrock, Michael
Project Reports:2013 Annual Report
2012 Annual Report
2011 Annual Report
Published Journal
Articles USDA
ARS (NP 108):
Non-journal publications:
Media for the aerobic growth of campylobacter - (Abstract Only)
Hinton Jr, A. 2013. Media for the aerobic growth of campylobacter. Southern Poultry Science Society Meeting Abstracts.
Microbiomic investigation of the poultry production chain: Using next generation sequencing to study general microbial and foodborne pathogen dynamics - (Abstract Only)
Accepted Publication (02-Oct-12)
Aerobic growth of campylobacter in media supplemented with a-ketoglutaric, lactic, and/or fumaric acids - (Proceedings)
Hinton Jr, A. 2012. Aerobic growth of campylobacter in media supplemented with a-ketoglutaric, lactic, and/or fumaric acids. World's Poultry Science Journal. 68(1):256-258.
Ability of lactate and pyruvate to stimulate aerobic growth of campylobacter in media supplemented with fumarate - (Abstract Only)
Hinton Jr, A. 2012. Ability of lactate and pyruvate to stimulate aerobic growth of campylobacter in media supplemented with fumarate [abstract]. Poultry Science Association Meeting Abstract.
Rapid molecular pathotyping of major salmonella enterica serotypes based on single-nucleotide polymorphisms (SNPs) in the adenylate cyclase (cyaA) gene - (Abstract Only)
Accepted Publication (06-Mar-12)
Aerobic growth of campylobacter in media supplemented with glutamic, lactic, and/or fumaric acids - (Abstract Only)
Hinton Jr, A. 2012. Aerobic growth of campylobacter in media supplemented with glutamic, lactic, and/or fumaric acids. Annual Meeting of the Institute of Food Technologists [abstract].
Aerobic and anaerobic cecal bacterial flora of commercially processed broilers - (Abstract Only)
Hinton Jr, A., Ingram, K.D., Jenkins, M.B. 2011. Aerobic and anaerobic cecal bacterial flora of commercially processed broilers. International Poultry Scientific Forum.
Effect of volumetric water content and crimson clover (trifolium incarnatum)growth on the survival of escherichia coli o157:h7 in a soil matrix - (Abstract Only)
Rothrock Jr, M.J., Frantz, J., Bumett, S., Cook, K.L. 2011. Effect of volumetric water content and crimson clover (trifolium incarnatum)growth on the survival of escherichia coli o157:h7 in a soil matrix [abstract]. ASA-CSSA-SSSA Annual Meeting Abstracts.
Bactericidal activity of alkaline salts of fatty acids towards bacteria associated with poultry processing - (Proceedings)
Hinton Jr, A., Ingram, K.D. 2011. Bactericidal activity of alkaline salts of fatty acids towards bacteria associated with poultry processing. XXII Latin American Poultry Congress, September 5-9, 2011, Buenos Aires, Argentina. Meeting Proceedings. #156.
Effect of pH alkaline salts of fatty acids on the inhibition of bacteria associated with poultry processing - (Abstract Only)
Hinton Jr, A., Ingram, K.D. 2011. Effect of pH alkaline salts of fatty acids on the inhibition of bacteria associated with poultry processing [abstract]. Poultry Science Meeting. 90(E-1):22.
Acidification delays the microbial mineralization of organic N forms in poultry litter - (Popular Publication)
Rothrock Jr, M.J., Cook, K.L., Warren, J.G., Eiteman, M.A., Sistani, K.R. 2010. Acidification delays the microbial mineralization of organic N forms in poultry litter. CSA News. 55(10):11-13.
Food Safety Categories:Sanitation and Pathogen Control
Government Policy and Regulations
Methodology
Farm-to-Table categories:Food processing
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