|Title:||Pathogen Mitigation in Livestock and Red Meat Production|
|Objective:||The overall goal of this project is to increase the microbiological safety and quality of red meat by reducing or eliminating foodborne pathogens. Accomplishment of this goal will require basic and applied research objectives targeted throughout the forkto-farm food-chain. |
Although this project has in the past and will always provide assistance to all three areas of red meat food safety (i.e., beef, lamb, and pork), the research proposed herein is weighted heavily towards beef as recent increases in recalls and illness outbreaks have been particularly detrimental to the beef industry.
This project focuses on the three primary red meat-related foodborne pathogens: Escherichia coli O157:H7, non-O157 Shiga toxin-producing E. coli (STEC), and Salmonella. E. coli O157:H7 receives the bulk of the research attention as it is the highest food safety priority of the beef industry. The three objectives of this project address needs in antimicrobial intervention, pathogen detection, and host-pathogen interaction. Experiments are proposed to develop and evaluate novel interventions for pathogen mitigation for the live animal in production and lairage environments, for carcasses at multiple stages of processing, and for the finished product.
In conjunction with intervention development, improvements to detection methodology and pathogen source identification are proposed for cattle production and processing, as well as the finished product. Finally, basic research of host-pathogen interactions will provide the basis for future developments in pathogen detection and intervention.
|More Info:||The meat processing industry will gain additional tools in the fight against pathogen contamination of carcasses and, ultimately, retail meat products. FSIS will receive pertinent information on which to base food safety policies and regulations. Successful completion of the objectives will benefit processors by increasing their ability to monitor production and develop improved interventions to minimize contamination and product loss, and will benefit consumers by decreasing the risk of foodborne illness.|
|Funding Source:||United States Department of Agriculture (USDA), Agricultural Research Service (ARS)|
|Institutions:||USDA/ARS - Northern Plains Area|
Wheeler, Tommy Lee
|Project Reports:||2011 Annual Report|
|Pending USDA ARS Publications (NP 108):|
Effects of in-plant interventions on reduction of enterohemorrhagic Escherichia coli and background indicator microorganisms on veal calf hides
ARS (NP 108):
Characterization of E. coli O157:H7 strains resulting from contamination of raw beef trim during high event periods
Arthur TM, Bono JL, Kalchayanand N .
Appl Environ Microbiol. 2014 Jan;80(2):506-14.
Impact of sampling area and location on measurement of indicator organisms during beef carcass interventions
Wang R, King DA, Koohmaraie M, Bosilevac JM .
J Food Prot. 2013 Dec;76(12):2069-73.
Mixed biofilm formation by Shiga Toxin-Producing Escherichia coli and Salmonella enterica Serovar Typhimurium enhanced bacterial resistance to sanitization due to extracellular polymeric substances
Wang R, Kalchayanand N, Schmidt JW, Harhay DM.
J Food Prot. 2013 Sep;76(9):1513-22.
Characterization E.coli 0157:H7 strains isolated from super-shedding cattle
Arthur TM, Ahmed R, Chase-Topping M, Kalchayanand N, Schmidt JW, Bono JL.
Appl Environ Microbiol. 2013 Jul;79(14):4294-303.
The physiologic state of Escherichia coli O157:H7 does not affect its detection in two commercial real-time PCR-based tests
Wang R, Schmidt JW, Arthur TM, Bosilevac JM.
Food Microbiol. 2013 Apr;33(2):205-12.
Resistance of various shiga toxin-producing Escherichia coli to electrolyzed oxidizing water
Ravirajsinh J, Hung Y-C, Bosilevac J.
Food Control. 2013 Apr;30(2):580-4.
Chromogenic agar medium for detection and isolation of Escherichia coli serogroups O26, O45, O103, O111, O121, and O145 from fresh beef and cattle feces
Kalchayanand N, Arthur TM, Bosilevac JM, Wells JE, Wheeler TL.
J Food Prot. 2013 Feb;76(2):192-9.
Detection of Escherichia coli O157:H7 and Salmonella enterica in air and droplets at three U.S. commercial beef processing plants
Schmidt JW, Arthur TM, Bosilevac JM, Kalchayanand N, Wheeler TL.
J Food Prot. 2012 Dec;75(12):2213-8.
Predicting the presence of non-O157 Shiga toxin-producing Escherichia coli in ground beef by using molecular tests for Shiga toxins, intimin, and O serogroups
Bosilevac JM, Koohmaraie M.
Appl Environ Microbiol. 2012 Oct;78(19):7152-5.
Evaluation of commonly used antimicrobial interventions for fresh beef inoculated with Shiga toxin-producing Escherichia coli serotypes O26, O45, O103, O111, O121, O145, and O157:H7
Kalchayanand N, Arthur TM, Bosilevac JM, Schmidt JW, Wang R, Shackelford SD, Wheeler TL.
J Food Prot. 2012 Jul;75(7):1207-12.
Efficacy of hypobromous acid as a hide-on carcass antimicrobial intervention
Schmidt JW, Wang R, Kalchayanand N, Wheeler TL, Koohmaraie M.
J Food Prot. 2012 May;75(5):955-8.
Microbiological analysis of bovine lymph nodes for the detection of Salmonella enterica
Brichta-Harhay DM, Arthur TM, Bosilevac JM, Kalchayanand N, Schmidt JW, Wang R, Shackelford SD, Loneragan GH, Wheeler TL.
J Food Prot. 2012 May;75(5):854-8.
Prevalence, enumeration, serotypes, and antimicrobial resistance phenotypes of salmonella enterica isolates from carcasses at two large United States pork processing plants
Schmidt JW, Brichta-Harhay DM, Kalchayanand N, Bosilevac JM, Shackelford SD, Wheeler TL, Koohmaraie M.
Appl Environ Microbiol. 2012 Apr;78(8):2716-26.
Detection methods and intervention strategies for shiga toxin-producing E. coli in beef - (Abstract Only)
Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Wheeler, T.L. 2012. Detection methods and intervention strategies for shiga toxin-producing E. coli in beef [abstract]. Annual Meeting of the Institute of Food Technologists. 061-01:52.
Efficacy of ultraviolet light (UV) and UV-ozone as an intervention to reduce pathogens-contaminated fresh beef - (Abstract Only)
Kalchayanand, N., Bosilevac, J.M., Wheeler, T.L. 2012. Efficacy of ultraviolet light (UV) and UV-ozone as an intervention to reduce pathogens-contaminated fresh beef.[Abstract] Annual Meeting of the Institute of Food Technologists.June 25-28, 2012, Las Vegas, Nevada. Abstract 035-71:25.
Analysis of E. coli O157:H7 strains isolated from raw beef contamination events - (Abstract Only)
Arthur, T.M. 2012. Analysis of E. coli O157:H7 strains isolated from raw beef contamination events [abstract]. 2012 Beef Industry Safety Summit. Abstract No. 11.
Characterization of E. coli O157:H7 strains associated with super-shedding cattle - (Abstract Only)
Arthur, T.M. 2012. Characterization of E. coli O157:H7 strains associated with super-shedding cattle [abstract]. 2012 Beef Industry Safety Summit. Abstract No. 1.
Prevalence and characterization of non-O157 Shiga toxin-producing Escherichia coli isolated from feedlot and cull dairy and beef herd cattle - (Abstract Only)
Accepted Publication (31-Jan-12)
Salmonella in lymph nodes of cattle presented for harvest - (Abstract Only)
Gragg, S.E., Loneragan, G.H., Brashears, M.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wang, R., Schmidt, J.W., Brooks, J.C., Shackelford, S.D., Wheeler, T.L., Brown, T.R., Brichta-Harhay, D.M. 2011. Salmonella in lymph nodes of cattle presented for harvest [abstract]. Proceeding of the 2011 Conference of Research Workers in Animal Diseases, December 4-6, 2011, Chicago, Illinois. p. 149.
Non-O157 Shiga toxin-producing Escherichia coli: prevalence associated with meat animals and controlling interventions - (Proceedings)
Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Wheeler, T.L. 2012. Non-O157 Shiga toxin-producing Escherichia coli: prevalence associated with meat animals and controlling interventions. Proceedings of American Meat Science Association 64th Annual Reciprocal Meat Conference, June 19-22, 2011, Kansas State University, Manhattan Kansas. p.1-9.
|Food Safety Categories:||Sanitation and Pathogen Control|
Government Policy and Regulations
|Farm-to-Table categories:||Food processing|
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