Food Safety Research Information Office
Title:Prevention and Characterization of Persistent Colonization by Escherichia coli O157:H7 and Other Shiga Toxin-Producing E. Coli (STEC) in Cattle
Objective:1) Determine and characterize molecular mechanisms promoting colonization, effective adherence, and persistence of E. coli O157:H7 and other STECs in cattle

2) Understand the impact of bovine intestinal environment and immune responses on growth, adherence, and persistence of E. coli O157:H7 and other STECs in cattle

3) Conduct comparative analysis of bovine E. coli O157:H7 and STEC isolates of public health significance to identify components for use in developing rapid diagnostic tools and effective interventions; and

4) Develop and test efficacy of chemical, biological, subunit proteins, and whole cell vaccines to prevent or reduce colonization of cattle intestines by E. coli O157:H7 and STECs.

More Info:
Approach:
Experimental animal models, tissue cultures, and specific mutants will be used to describe molecular mechanism(s) enabling E. coli O157:H7 bacteria to grow, adhere, and colonize the cattle intestine. Reporter gene fusions and global gene analysis technologies will be used to determine effects of host gastrointestinal environment and innate immune system on the expression of specific bacterial genetic systems and metabolic pathways that promote E. coli O157:H7 persistence in cattle intestine. Emerging non-O157 STEC serotypes will be compared with E. coli O157:H7 to identify genetic and molecular features unique to these serotypes. Bacterial genes or gene products identified in these studies will be used, based on their importance in colonization, for developing whole-cell or protein/subunit protein vaccines for reducing or eliminating E. coli O157:H7 and non-O157 STEC colonization and shedding in cattle.
Funding Source:United States Department of Agriculture (USDA), Agricultural Research Service (ARS)
Type:Appropriated
Start Date:2010
End Date:2015
Project Number:3625-32000-100-00
Accession Number:420815
Institutions:USDA/ARS - Midwest Area
Investigators:Casey, Thomas
Kudva, Indira
Sharma, Vijay
Stanton, Thaddeus
Project Reports:2013 Annual Report
2012 Annual Report
2011 Annual Report
Published Journal
Articles USDA
ARS (NP 108):
Escherichia coli O157:H7 lacking qseBC encoded quorum sensing system outcompetes the parental strain in colonization of cattle intestine
Sharma VK, Casey T .
Appl Environ Microbiol. 2014 Mar;80(6):1882-92.
The Escherichia coli O157:H7 bovine rumen fluid proteome reflects adaptive bacterial responses
Kudva IT, Stanton TB, Lippolis JD.
BMC Microbiol. 2014 Feb 21;14(1):48.
Determining the relative contribution and hierarchy of qseBC and hha in the regulation of flagellar motility of Escherichia coli O157:H7
Sharma VK, Casey T.
PLoS One. 2014 Jan 21;9(1):e85866.
Bison and bovine rectoanal junctions exhibit similar cellular architecture and Escherichia coli O157 adherence patterns
Kudva IT, Stasko JA.
BMC Vet Res. 2013 Dec 28;9(1):266.
Polymorphic amplified typing sequences (PATS) strain typing system accurately discriminates a set of temporally and spatially disparate Escherichia coli (O157) isolates associated with human infection
Kudva IT, Smole S, Griffin RW, Garren J, Kalia N, Murray M, John M, Timperi R, Calderwood SB .
Open Microbiol J. 2013 Oct;7:123-9.
Fluorescence spectroscopy of the retina from scrapie-infected mice
Bose S, Schönenbrücher H, Richt JA, Casey TA, Rasmussen MA, Kehrli ME Jr, Petrich JW.
Photochem Photobiol. 2013 Jul;89(4):864-8.
Evaluation of the impact of quorum sensing transcriptional regulator SdiA on long-term persistence and fecal shedding of Escherichia coli O157:H7 in weaned calves
Sharma VK, Bearson SM .
Microb Pathog. 2013 Apr;57:21-6.
Hha controls Escherichia coli O157:H7 biofilm formation by differential regulation of global transcriptional regulators FlhDC and CsgD
Sharma VK, Bearson BL.
Appl Environ Microbiol. 2013 Apr;79(7):2384-96.
Adherence of non-O157 Shiga toxin-producing Escherichia coli to bovine recto-anal junction squamous epithelial cells appears to be mediated by mechanisms distinct from those used by O157
Kudva IT, Hovde CJ, John M.
Foodborne Pathog Dis. 2013 Apr;10(4):375-81.
Evaluation of heat-labile enterotoxins type IIa and type IIb in the pathogenicity of enterotoxigenic Escherichia coli for neonatal pigs
Casey TA, Connell TD, Holmes RK, Whipp SC.
Vet Microbiol. 2012 Sep 14;159(1):83-9.
Proteins other than the locus of enterocyte effacement-encoded proteins contribute to Escherichia coli O157:H7 adherence to bovine rectoanal junction stratified squamous epithelial cells
Kudva IT, Griffin RW, Krastins B, Sarracino DA, Calderwood SB, John M.
BMC Microbiol. 2012 Jun 12;12:103.
Correlating levels of type III secretion and secreted proteins with fecal shedding of Escherichia coli O157:H7 in cattle
Sharma VK, Sacco RE, Kunkle RA, Bearson SM, Palmquist DE.
Infect Immun. 2012 Apr;80(4):1333-42.
In vitro adherence patterns of Shigella serogroups to bovine recto-anal junction squamous epithelial (RSE) cells are similar to those of Escherichia coli O157
Kudva IT.
Foodborne Pathog Dis. 2012 Apr;9(4):346-51.
Polymorphic amplified typing sequences and pulsed-field gel electrophoresis yield comparable results in the strain typing of a diverse set of bovine Escherichia coli O157:H7 isolates
Kudva IT, Davis MA, Griffin RW, Garren J, Murray M, John M, Hovde CJ, Calderwood SB.
Int J Food Microbiol. 2012;2012:140105.
A tripartite fusion, FaeG-FedF-LT(192)A2:B, of enterotoxigenic Escherichia coli (ETEC) elicits antibodies that neutralize cholera toxin, inhibit adherence of K88 (F4) and F18 fimbriae, and protect pigs against K88ac/heat-labile toxin infection
Ruan X, Liu M, Casey TA, Zhang W.
Clin Vaccine Immunol. 2011 Oct;18(10):1593-9.
Non-Journal Publications:
Genomic and molecular analysis of the hyperadherent phenotype of an Escherichia coli O157:H7 super shedder isolate - (Abstract Only)
Moreau, M.R., Cote, R., Katani, R., Kudva, I.T., Carter, M.Q., Brandl, M., Kapur, V. 2013. Genomic and molecular analysis of the hyperadherent phenotype of an Escherichia coli O157:H7 super shedder isolate [abstract]. In: Proceedings of Molecular Biology, Sensing and Signaling Across the Mucosa: From Homeostasis to Pathogenesis, June 4-6, 2013, University Park, Pennsylvania. p. 92.
Proteomic analysis of Escherichia coli O157 cultured in bovine rumen fluid - (Abstract Only)
Kudva, I.T., Stanton, T.B., Lippolis, J.D. 2013. Proteomic analysis of Escherichia coli O157 cultured in bovine rumen fluid [abstract]. 113th General Meeting, American Society for Microbiology. p. 190.
Escherichia coli O157 adherence to the bovine recto-anal junction (RAJ) squamous epithelial cells is mediated by adhesins other than those encoded by genes on the Locus of Enterocyte Attachment (LEE) pathogenicity island - (Abstract Only)
Kudva, I.T., Stasko, J.A. 2012. Escherichia coli O157 adherence to the bovine recto-anal junction (RAJ) squamous epithelial cells is mediated by adhesins other than those encoded by genes on the Locus of Enterocyte Attachment (LEE) pathogenicity island [abstract]. American Society for Microbiology General Meeting, June 16-19, 2012, San Francisco, California. p. 215.
Quorum sensing transcriptional regulator QseA is essential for the expression of multiple virulence regulons of enterohemorrhagic Escherichia coli O157:H7 - (Abstract Only)
Sharma, V.K. 2012. Quorum sensing transcriptional regulator QseA is essential for the expression of multiple virulence regulons of enterohemorrhagic Escherichia coli O157:H7 [abstract]. In: Proceedings of the 8th International Symposium on Shiga Toxin (Verocytotoxin) Producing Escherichia Coli. May 5-9, 2012, Amsterdam, The Netherlands. 59:8.
Quorum sensing transcriptional regulator QseA is essential for the expression of multiple virulence regulons of enterohemorrhagic Escherichia coli O157:H7 - (Proceedings)
Sharma, V.K. 2012. Quorum sensing transcriptional regulator QseA is essential for the expression of multiple virulence regulons of enterohemorrhagic Escherichia coli O157:H7. Zoonoses and Public Health. 59:(Suppl. 1), 2-18.
Enterohemorrhagic Escherichia coli O157:H7 requires quorum sensing transcriptional regulators QseA and SdiA for colonization and persistence in the bovine intestinal tract - (Abstract Only)
Sharma, V.K., Bearson, S.M., Thacker, T.C. 2011. Enterohemorrhagic Escherichia coli O157:H7 requires quorum sensing transcriptional regulators QseA and SdiA for colonization and persistence in the bovine intestinal tract [abstract]. 71st Annual Meeting of the North Central Branch of the American Society for Microbiology. October 7-8, 2011, Des Moines, Iowa. 59:147.
Definition of the Escherichia coli O157:H7 proteome under nutrient-limiting conditions to identify targets for efficacious cattle vaccines - (Abstract Only)
Kudva, I.T., Griffin, R.W., Krastins, B., Sarracino, D.A., Calderwood, S.B., John, M. 2011. Definition of the Escherichia coli O157:H7 proteome under nutrient-limiting conditions to identify targets for efficacious cattle vaccines [abstract]. 71st Annual Meeting of the North Central Branch of the American Society for Microbiology. October 7-8, 2011, Des Moines,Iowa. 62:149.
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