Food Safety Research Information Office
Title:Molecular Analysis of Salmonella Virulence, Antibiotic Resistance, and Host Response

1)Identify molecular mechanisms in Salmonella that impact virulence and antimicrobial resistance in the gastrointestinal environment of cattle and swine.

2)Characterize the genetic pathways in swine that respond to Salmonella infection, their relationship to carrier status, and the ability to predict and control Salmonella prevalence and ultimately food safety.

3)Investigate interactions between Salmonella and host microbiota that influence Salmonella colonization and persistence, alter the commensal composition in the host, and provide novel intervention strategies to decrease Salmonella in swine populations.

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1) Use phenotypic assays, recombineering, and global transcriptional analysis to identify the genes and genetic pathways involved in regulating Salmonella DT104 hyper-invasion in response to a) rumen protozoa and b) sub-inhibitory concentrations of antibiotics.

2) Use an attenuated, LPS/flagella-deficient S. Typhimurium strain to re-focus the pig’s immune response from serovar-specific antigens to conserved antigens during vaccination against Salmonella, thereby providing cross-protection against multiple Salmonella serovars.

3) Classify the porcine gene expression differences between low and high shedders of Salmonella during colonization.

4) Compare the gastrointestinal microbial communities of pigs before, during and after Salmonella colonization to assess correlations with Salmonella shedding status in the pigs.

Funding Source:United States Department of Agriculture (USDA), Agricultural Research Service (ARS)
Start Date:2010
End Date:2015
Project Number:3625-32000-101-00
Accession Number:421023
Institutions:USDA/ARS - Midwest Area
Investigators:Bearson, Shawn
Brunelle, Brian
Stanton, Thaddeus
Project Reports:2013 Annual Report
2012 Annual Report
2011 Annual Report
Published Journal
Articles USDA
ARS (NP 108):
TLR4 single nucleotide polymorphisms (SNPs) associated with Salmonella shedding in pigs
Kich JD, Uthe JJ, Benavides MV, Cantao ME, Zanella R, Tuggle CK, Bearson SM.
J Appl Genet. 2014 Feb 25. [Epub ahead of print]
Polynucleotide phosphorylase (PNPase) is required for Salmonella enterica serovar Typhimurium colonization in swine
Bearson SM, Bearson BL, Lee IS, Kich JD.
Microb Pathog. 2013 Oct 11;65C:63-6.
Tetracycline accelerates the temporally-regulated invasion response in specific isolates of multidrug-resistant Salmonella enterica serovar Typhimurium
Brunelle BW, Bearson SM, Bearson BL.
BMC Microbiol. 2013 Sep 11;13(1):202.
Food Safety Categories:On-Farm Food Safety
Government Policy and Regulations
Pathogen Biology
Farm-to-Table Categories:On-farm food production
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