|Title:||Innovative Materials for Use in Mycotoxin Detection|
Assays for detecting low molecular weight toxins rely upon components that can interact with the toxins and facilitate toxin isolation or detection. Because of this, the goal of developing assays with better performance characteristics (such as sensitivity, speed, and robustness) requires the development of improved materials that bind the toxins. To meet this goal, we propose four objectives:
Objective 1: Develop novel biologically-based materials that bind foodborne toxicants;
Objective 2: Design and develop synthetic-based materials for detection of agricultural and food-related toxins;
Objective 3: Develop computational methods for foodborne toxins that enable new strategies to reduce exposure; Objective 4: Develop detection strategies for emerging toxins and toxins of commercial importance.
Toxins produced by fungi, mycotoxins, can cause devastating economic effects by affecting the safety and marketability of grain, and by causing disease in livestock. Diagnosis of health problems caused by mycotoxins is often difficult because while certain of the toxins can cause acute disease, most cause sub-acute or chronic effects that are more difficult to discern. For these reasons, attempts are made to detect mycotoxins at the many stages from crop production to finished product. As a result, a vital part of mycotoxin control is the availability of rapid, accurate, sensitive, and cost effective methods for toxin detection and quantitation. Fortunately, many such methods exist and are commercially available. With a few exceptions, most of these methods rely, in some fashion, on components that bind the toxins. Ideally, the binding components are highly specific for the toxins of interest and are capable of performing under their expected conditions of use. As analytical technologies progress, assays are required to perform under increasingly demanding conditions, requiring advances in their component materials. We propose to apply the expertise of the investigators in synthetic chemistry, antibody development, immunoassay and sensor development, and instrumental assay methodology, toward the development of the next generation of toxin binding materials, such materials being the fundamental basis of improved analytical technologies for these toxins. By improving technologies for detecting natural toxins, this project will have a direct impact on the ability of producers, processors, and regulatory agencies to improve monitoring programs for natural toxins, and thereby improve the safety of the U.S. food supply.
|Funding Source:||United States Department of Agriculture (USDA), Agricultural Research Service (ARS)|
|Institutions:||USDA/ARS - Midwest Area|
|Project Reports:||2013 Annual Report|
2012 Annual Report
2011 Annual Report
ARS (NP 108):
Fluorescence polarisation immunoassays for rapid, accurate and sensitive determination of mycotoxins
Lippolis V, Maragos C.
World Mycotoxin J. 2014 Jun 5. [Epub ahead of print]
Determination of deoxynivalenol in bran and whole-wheat flour by fluorescence polarization immunoassay
Valenzano S, Lippolis V, Pascale M, DeMarco C, Maragos C,Suman M,Visconti A .
J Food Anal Methods. 2014 Apr;7(4):806-13.
Production of anti-idiotype antibodies for deoxynivalenol and their evaluation with three immunoassay platforms
Mycotoxin Res. 2014 Feb 13;30(2):103-11.
Fluorescence polarization immunoassay for rapid, accurate and sensitive determination of ochratoxin A in wheat
Lippolis V, Pascale M, Valenzano S, Porricelli ACR, Suman M, Visconti A.
Food Anal Method. 2014 Feb;7(2):298-307.
Determination of fusaric acid in maize using molecularly imprinted SPE clean-up
Appell M, Jackson MA, Wang LC, Ho CH, Mueller A.
J Sep Sci. 2014 Feb;37(3):281-6.
Developments in mycotoxin analysis: an update for 2012-2013
Berthiller F, Burdaspal PA, Crews C, Iha MH, Krska R, Lattanzio VMT, MacDonald S, Malone RJ, Maragos C, Solfrizzo J, Whitaker TB.
World Mycotoxin J. 2014 Jan 24. [Epub ahead of print]
Determination of the aflatoxin AFB1 from corn by direct analysis in real time-mass spectrometry (DART-MS)
Busman M, Liu J, Zhong H, Bobell JR, Maragos CM.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2014;31(5):932-9.
Development and evaluation of monoclonal antibodies for the glucoside of T-2 toxin (T2-Glc)
Maragos CM, Kurtzman C, Busman M, Price N, McCormick S.
Toxins (Basel). 2013 Jul 19;5(7):1299-313.
Interactions between cyclodextrins and fluorescent T-2 and HT-2 toxin derivatives: a physico-chemical study
Ventrella A, Verrone R, Longobardi F, Agostiano A, Lippolis V, Pascale M, Maragos C, Appell M, Catucci L.
J Incl Phenom Macro. 2013 Apr;75(3):285-92.
Developments in mycotoxin analysis: an update for 2011-2012
Shephard G, Berthiller F, Burdaspal P, Crews C, Jonker M, Krska R, Lattanzio VMT, Macdonald S, Malone R, Maragos C.
World Mycotoxin J. 2013 Feb;6(1):3-30.
Use of cyclodextrin-based polymer for patulin analysis in apple juice
Shirasawa T, Ueda M, Appell MD, Goto T.
Sorption of ochratoxin A from aqueous solutions using β-cyclodextrin-polyurethane polymer
Appell M, Jackson MA.
Toxins. 2012 Feb;4(2):98-109.
Developments in mycotoxin analysis: an update for 2010 - 2011
Shephard G, Berthiller F, Burdaspal P, Crews C, Jonker M, Krska R, Macdonald S, Malone R, Maragos C, Sabino M, Solfrizzo M, Van Egmond H, Whitaker T.
World Mycotoxin J. 2012 Feb;5(1):3-30.
Signal amplification using colloidal gold in a biolayer interferometry-based immunosensor for the mycotoxin deoxynivalenol
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2012;29(7):1108-17.
Zearalenone occurrence in surface waters in central Illinois, USA
Food Addit Contamin Part B. 2012;5(1):55-64.
Production and characterization of a single chain variable fragment (scFv) against the mycotoxin deoxynivalenol
Maragos C, Li L, Chen D.
Food Agric Immunol. 2012;23(1):51-67.
Measurement of trichothecene mycotoxins in wheat using a biolayer interferometry-based biosensor - (Abstract Only)
Detection of â€œmaskedâ€ trichothecenes by immunoassay - (Abstract Only)
â€œMaskedâ€ mycotoxin detection: What is a poor chemist to do? - (Abstract Only)
Preface - (Book / Chapter)
Appell, M.D., Jackson, M.A. 2014. Preface. In: Park, B; Appell, M., Eds. ACS Symposium #1143: Advances in Applied Nanotechnology for Agriculture. Washington, DC: American Chemical Society. p. ix.
Current and state-of-the-art approaches for detecting mycotoxins in commodities - (Abstract Only)
Maragos, C.M., Busman, M. 2013. Current and state-of-the-art approaches for detecting mycotoxins in commodities. Meeting Abstract. p. 22.
Applications of Nanoporous Materials in Agriculture - (Book / Chapter)
Accepted Publication (22-Apr-13)
Adsorption of mycotoxins in beverages onto functionalized mesoporous silicas - (Abstract Only)
Accepted Publication (11-Apr-13)
Materials used in toxin detection, from A to Z - (Abstract Only)
Maragos, C.M. 2012. Materials used in toxin detection, from A to Z. Meeting Abstract.
Synthetic materials to reduce exposure to mycotoxins in fermented foods and beverages - (Abstract Only)
Appell, M.D., Jackson, M.A. 2012. Synthetic materials to reduce exposure to mycotoxins in fermented foods and beverages. Food Micro 2012 International Symposium Food Microbiology and Hygiene. p. 69.
Quantum chemical studies of estrogenic compounds - (Book / Chapter)
Bosma, W.B., Appell, M.D. 2013. Quantum chemical studies of estrogenic compounds. In: Pietro Cozzini, G., Kellogg, G.E., editors. Computational Approaches to Nuclear Receptors. Cambridge, UK: The Royal Society of Chemistry. p. 110-116.
Evaluation of functionalized SBA-15 to reduce patulin levels in apple juice - (Abstract Only)
Appell, M.D., Jackson, M.A. 2012. Evaluation of functionalized SBA-15 to reduce patulin levels in apple juice [abstract]. American Chemical Society. AGFD 244.
Physicochemical study on interactions between T-2 and HT-2 toxin derivatives and cyclodextrins - (Abstract Only)
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