|Title:||Innovative Materials for Use in Mycotoxin Detection|
Toxins produced by fungi, mycotoxins, can cause devastating economic effects by affecting the safety and marketability of grain, and by causing disease in livestock. Diagnosis of health problems caused by mycotoxins is often difficult because while certain of the toxins can cause acute disease, most cause sub-acute or chronic effects that are more difficult to discern.
For these reasons, attempts are made to detect mycotoxins at the many stages from crop production to finished product. As a result, a vital part of mycotoxin control is the availability of rapid, accurate, sensitive, and cost effective methods for toxin detection and quantitation. Fortunately, many such methods exist and are commercially available. With a few exceptions, most of these methods rely, in some fashion, on components that bind the toxins. Ideally, the binding components are highly specific for the toxins of interest and are capable of performing under their expected conditions of use. As analytical technologies progress, assays are required to perform under increasingly demanding conditions, requiring advances in their component materials.
We propose to apply the expertise of the investigators in synthetic chemistry, antibody development, immunoassay and sensor development, and instrumental assay methodology, toward the development of the next generation of toxin binding materials, such materials being the fundamental basis of improved analytical technologies for these toxins.
By improving technologies for detecting natural toxins, this project will have a direct impact on the ability of producers, processors, and regulatory agencies to improve monitoring programs for natural toxins, and thereby improve the safety of the U.S. food supply.
|More Info:||Monitoring and control of mycotoxins in commodities and foods is essential for reducing human and animal exposure to these toxins. Isolation and detection of toxins rely heavily upon toxin binding materials. As such, attaining this project's objectives will allow the development of more rapid and sensitive methods that can be used for better control of mycotoxins at various stages in the supply chain through diversion of contaminated materials. Additionally, some of the binding materials from this work may have the potential to remove mycotoxins from contaminated products, thereby improving the safety and value of the commodity.|
|Funding Source:||United States Department of Agriculture (USDA), Agricultural Research Service (ARS)|
|Institutions:||USDA/ARS - Midwest Area|
|Pending USDA ARS Publications (NP 108):|
ARS (NP 108):
Development and evaluation of monoclonal antibodies for the glucoside of T-2 toxin (T2-Glc)
Maragos CM, Kurtzman C, Busman M, Price N, McCormick S.
Toxins (Basel). 2013 Jul 19;5(7):1299-313.
Determination of deoxynivalenol in bran and whole-wheat flour by fluorescence polarization immunoassay
Valenzano S, Lippolis V, Pascale M, DeMarco C, Maragos C,Suman M,Visconti A .
J Food Anal Methods. 2013 Jul. [Epub ahead of print]
Interactions between cyclodextrins and fluorescent T-2 and HT-2 toxin derivatives: a physico-chemical study
Ventrella A, Verrone R, Longobardi F, Agostiano A, Lippolis V, Pascale M, Maragos C, Appell M, Catucci L.
J Incl Phenom Macro. 2013 Apr;75(3):285-92.
Developments in mycotoxin analysis: an update for 2011-2012
Shephard G, Berthiller F, Burdaspal P, Crews C, Jonker M, Krska R, Lattanzio VMT, Macdonald S, Malone R, Maragos C.
World Mycotoxin J. 2013 Feb;6(1):3-30.
Use of cyclodextrin-based polymer for patulin analysis in apple juice
Shirasawa T, Ueda M, Appell MD, Goto T.
Sorption of ochratoxin A from aqueous solutions using β-cyclodextrin-polyurethane polymer
Appell M, Jackson MA.
Toxins. 2012 Feb;4(2):98-109.
Developments in mycotoxin analysis: an update for 2010 - 2011
Shephard G, Berthiller F, Burdaspal P, Crews C, Jonker M, Krska R, Macdonald S, Malone R, Maragos C, Sabino M, Solfrizzo M, Van Egmond H, Whitaker T.
World Mycotoxin J. 2012 Feb;5(1):3-30.
Signal amplification using colloidal gold in a biolayer interferometry-based immunosensor for the mycotoxin deoxynivalenol
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2012;29(7):1108-17.
Zearalenone occurrence in surface waters in central Illinois, USA
Food Addit Contamin Part B. 2012;5(1):55-64.
Production and characterization of a single chain variable fragment (scFv) against the mycotoxin deoxynivalenol
Maragos C, Li L, Chen D.
Food Agric Immunol. 2012;23(1):51-67.
Current and state-of-the-art approaches for detecting mycotoxins in commodities - (Abstract Only)
Maragos, C.M., Busman, M. 2013. Current and state-of-the-art approaches for detecting mycotoxins in commodities. Meeting Abstract. p. 22.
Applications of Nanoporous Materials in Agriculture - (Book / Chapter)
Accepted Publication (22-Apr-13)
Adsorption of mycotoxins in beverages onto functionalized mesoporous silicas - (Abstract Only)
Accepted Publication (11-Apr-13)
Materials used in toxin detection, from A to Z - (Abstract Only)
Maragos, C.M. 2012. Materials used in toxin detection, from A to Z. Meeting Abstract.
Synthetic materials to reduce exposure to mycotoxins in fermented foods and beverages - (Abstract Only)
Appell, M.D., Jackson, M.A. 2012. Synthetic materials to reduce exposure to mycotoxins in fermented foods and beverages. Food Micro 2012 International Symposium Food Microbiology and Hygiene. p. 69.
Quantum chemical studies of estrogenic compounds - (Book / Chapter)
Bosma, W.B., Appell, M.D. 2013. Quantum chemical studies of estrogenic compounds. In: Pietro Cozzini, G., Kellogg, G.E., editors. Computational Approaches to Nuclear Receptors. Cambridge, UK: The Royal Society of Chemistry. p. 110-116.
Evaluation of functionalized SBA-15 to reduce patulin levels in apple juice - (Abstract Only)
Appell, M.D., Jackson, M.A. 2012. Evaluation of functionalized SBA-15 to reduce patulin levels in apple juice [abstract]. American Chemical Society. AGFD 244.
Physicochemical study on interactions between T-2 and HT-2 toxin derivatives and cyclodextrins - (Abstract Only)
|Food Safety Categories:||Government Policy and Regulations|
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