|Title:||Pathogen Detection and Intervention Methods for Shellfish|
|Objective:||The greatest obstacles to seafood safety and the safety of aquaculture products are enteric viruses and the vibrio bacteria, which together cause the vast majority of seafood-related illnesses and deaths in the U.S. each year. We will explore mechanisms by which viruses and vibrios accumulate and persist within oysters and will develop processing interventions against these pathogens. |
Vibrio efforts will compare the effects of genetic and environmental factors on the uptake, persistence, and depletion of pandemic V. parahaemolyticus O3:K6, and other virulent and avirulent strains of V. parahaemolyticus and V. vulnificus in oysters. We will also isolate and characterize bacterial viruses (bacteriophages) which infect fish and human pathogenic vibrios (e.g., V. tubiashii, V. parahaemolyticus and V. vulnificus) with the goal of developing phage-based processing strategies to eliminate these pathogens from shellfish.
Enteric virus studies will focus on developing enhanced detection techniques, evaluating mitigation strategies, and identifying mechanisms of virus persistence within shellfish. Virus detection research will seek methods to discriminate between infectious and inactivated viruses as well as to simplify, streamline, and automate shellfish extraction protocols for viruses (e.g., hepatitis A virus and human norovirus).
Intervention strategies for hepatitis A and E viruses, and noroviruses will focus on alternative technologies, such as non-thermal processing techniques (e.g., high pressure and electron beam irradiation) with the ultimate goal to mitigate shellfish contamination and illness, and to reduce financial losses to the shellfish aquaculture industry.
This project provides an integrated approach to address vibrio and virus issues relevant to industry, regulatory, and consumer needs.
|More Info:||This work is designed to reduce impediments to the shellfish industry and to reduce illness among shellfish consumers.|
|Funding Source:||United States Department of Agriculture (USDA), Agricultural Research Service (ARS)|
|Institutions:||USDA/ARS - North Atlantic Area|
ARS (NP 108):
The influence of temperature, pH, and water immersion on the high hydrostatic pressure inactivation of GI.1 and GII.4 human noroviruses
Li X, Chen H, Kingsley DH.
Int J Food Microbiol. 2013 Oct;167(2):138–43.
Sensitivity of hepatitis A and murine norovirus to electron beam irradiation in oyster homogenates and whole oysters - quantifying the reduction in potential infection risks
Nair C, Dancho BA, Kingsley DH, Calci K, Meade GK, Mena KD, Pillai S .
Appl Environ Microbiol. 2013 Jun;79(12):3796-801.
Susceptibility of murine norovirus and hepatitis A virus to electron beam irradiation in oysters and quantifying the reduction in potential infection risks
Praveen C, Dancho BA, Kingsley DH, Calci KR, Meade GK, Mena KD, Pillai SD.
Appl Environ Microbiol. 2013 Jun;79(12):3796-801.
Murine macrophage inflammatory cytokine production and immune activation in response to Vibrio parahaemolyticus infection
Waters S, Luther S, Joerger T, Richards GP, Boyd EF, Parent MA.
Microbiol Immunol. 2013 Apr;57(4):323-8.
Lack of Norovirus Replication and Histo-Blood Group Antigen Expression in 3-Dimensional Intestinal Epithelial Cells
Herbst-Kralovets MM, Radtke AL, Lay MK, Bolick AN, Sarker SS, Kingsley DH, Arntzen CJ, Estes MK, Nickerson C.
Emerg Infect Diseases. 2013 Mar;19(3):431-8.
High pressure processing and its application to the challenge of virus-contaminated foods
Food Environ Virol. 2013 Mar;5(1):1-12.
Resilience of norovirus GII.4 to freezing and thawing: implications for virus infectivity
Richards GP, Watson MA, Meade GK, Hovan GL, Kingsley DH.
Food Environ Virol. 2012 Dec;4(4):192-7.
Predatory bacteria as natural modulators of Vibrio parahaemolyticus and Vibrio vulnificus in seawater and oysters
Richards GP, Fay JP, Dickens KA, Parent MA, Soroka DS, Boyd EF.
Appl Environ Microbiol. 2012 Oct;78(20):7455-66.
The Vibrio parahaemolyticus ToxRS regulator is required for stress tolerance and colonization in a novel orogastric streptomycin-induced adult murine model
Whitaker WB, Parent MA, Boyd A, Richards GP, Boyd EF.
Infect Immun. 2012 May;80(5):1834-45.
Critical review of norovirus surrogates in food safety research: rationale for considering volunteer studies
Food Environ Virol. 2012 Mar;4(1):6-13.
Shellfish-associated enteric virus illness: virus localization, disease outbreaks and prevention - (Book / Chapter)
Accepted Publication (28-Aug-13)
Detection of enteric viruses in shellfish - (Proceedings)
Richards, G.P., Cliver, D.O., Greening, G.E. 2013. Detection of enteric viruses in shellfish. Meeting Proceedings. G. Sauve, editor In Molluscan Shellfish Safety, Proceedings of the 8th International Conference on Molluscan Shellfish Safety, Charlottetown, Prince Edward Island, Canada, June 12-17, 2011.Springer, New York, pp. 177-183.
An introduction to food and waterborne viruses: diseases, transmission, outbreaks, detection and control - (Book / Chapter)
Cook, N., Richards, G.P. 2013. An introduction to food and waterborne viruses: diseases, transmission, outbreaks, detection and control. Book Chapter. Food and Waterborne Viruses, Woodhead Publishing Co, Cambridge, United Kingdom, pp.3-18.
Shellfish contamination and spoilage - (Book / Chapter)
Accepted Publication (01-Feb-13)
Novel methods for detection of foodborne viruses - (Abstract Only)
Kingsley, D.H. 2012. Novel methods for detection of foodborne viruses. Meeting Abstract., American Chemical Society (ACS) meeting., Philadelphia, PA., August 19-23, 2012., Volume 1, Page 1.
Recent intervention and detection advances for shellfish-borne norovirus - (Abstract Only)
Kingsley, D.H. 2012. Recent intervention and detection advances for shellfish-borne norovirus. Meeting Abstract. National Committee for the Microbiologist Criteria Subcommittee., Washington, DC., May 8-10,2012.Volume 1, Page 1.
Food safety research at Delaware State University: keeping the runs from slowing you down - (Abstract Only)
Kingsley, D.H. 2012. Food safety research at Delaware State University: keeping the runs from slowing you down. Meeting Abstract.Delaware State University, College of Agriculture and Related Sciences, Dover, DE, April 13, 2012., Volume 1, Page 1.
An extraction method for discrimination between infectious and inactive norovirus using RT-PCR - (Abstract Only)
Kingsley, D.H., Dancho, B. 2012. An extraction method for discrimination between infectious and inactive norovirus using RT-PCR. Meeting Abstract. American Society for Virology., Madison, Wisconsin., July 21-25, 2012., Volume 1, Page 1.
Foodborne viruses - (Book / Chapter)
Accepted Publication (23-Aug-11)
In memoriam Dean Otis Cliver 1935-2011 - (Other)
Richards, G.P. 2011. In memoriam Dean Otis Cliver 1935-2011. Food and Environmental Virology. 3(3):99-108. DOI: 10.1007/s12560-011-9064-7.
Sensitive detection of multiple hepatitis A virus genotypes with a single polony-based assay - (Abstract Only)
Accepted Publication (04-Mar-11)
|Food Safety Categories:||Food and Feed Handling and Processing|
Government Policy and Regulations
|Farm-to-Table categories:||On-farm food production|
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