|Title:||Detection and Typing of Foodborne Pathogens|
|Objective:||This research project will undertake the development of integrated, high-throughput, multiplex assay systems capable of simultaneously testing many food samples for the presence of multiple bacterial targets. |
A comprehensive approach will be taken addressing the challenges of isolating and concentrating multiple target pathogens, with intact genotype and phenotype elements, from food samples as well as their detection, quantitation, and typing. Protein and DNA microarray platforms will form the primary basis for detection as well as techniques capable of identifying target microorganisms at the sub-species level via novel typing strategies.
These strategies include generation of microarrays (and other multiplex-capable biosensor-based methods) containing: (1) very high numbers of unique nucleic acid probes or antibodies, (2) biorecognition elements selected from large repertoire libraries, and (3) commercially available typing antisera (before and after fractionation via chromatographic means).
In addition, other approaches will be investigated including: (1) optical light scattering of bacterial colonies combined with quantitative multiplex PCR and (2) enhanced genotyping via novel variations of existing methods. Detection and typing endeavors will be conducted in conjunction with research that seeks to rapidly concentrate select bacterial cells via physical means (e.g., novel filtration) and/or custom-developed selective growth enrichment methods that yield unaltered bacterial descendants. Delivery of intact and highly concentrated bacterial cell targets will facilitate reproducible detection and accurate characterization by typing methods. Emergent technologies will be directed towards usage by food producers and regulatory agencies for food safety/security monitoring as well as follow-up investigations.
Improved methods for the rapid detection and identification of pathogens are expected to have a significant impact on the prevention and control of economic loss, morbidity, and mortality associated with outbreaks of foodborne illness.
|More Info:||The proposed research will produce reliable, cost-effective, high-throughput, quantitative and qualitative measurements of microbial pathogens and/or toxins in food products. These multi-sample, multi-analyte detection tools are needed by food producers and regulators to ensure the safety of the food supply. In addition, these detection methodologies will provide data to carry out risk assessment, to develop and validate predictive microbial models, and to identify where intervention is most needed. This information will assist the implementation of Hazard Analysis and Critical Control Point (HACCP) programs by FSIS, FDA, and their regulated industries. With respect to food security, the developed biosensor detection and characterization technologies will allow ARS to strengthen and expand laboratory preparedness and to rapidly develop laboratory methods for the detection of select agents (microbial pathogens, toxins and potentially, chemical contaminants) in adulterated foods. In addition, effective methods will be transferred to the Federal Emergency Response Network (FERN).|
|Funding Source:||United States Department of Agriculture (USDA), Agricultural Research Service (ARS)|
|Institutions:||USDA/ARS - North Atlantic Area|
|Pending USDA ARS Publications (NP 108):|
ARS (NP 108):
Optimization and application of a custom microarray for the detection and genotyping of E. coli O157:H7 in fresh meat samples
Suo B, He Y, Irwin PL, Gehring AG .
Food Anal Methods. 2013 Oct;6(5):1477-84.
|Food Safety Categories:||Government Policy and Regulations|
|Farm-to-Table categories:||Food processing|
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